The Sugen/Hypoxia Rat Model for Pulmonary Hypertension and Right Heart Failure

Olympia Bikou, Yassine Sassi

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

Pulmonary hypertension (PH) is a devastating disease, characterized by complex remodeling of the pulmonary vasculature. PH is classified into five groups based on different etiology, pathology, as well as therapy and prognosis. Animal models are essential for the study of underlying mechanisms, pathophysiology, and preclinical testing of new therapies for PH. The complexity of the disease with different clinical entities dictates the necessity for more than one animal model to resemble PH, as a single model cannot imitate the broad spectrum of human PH. Here we describe a detailed protocol for creating a rat model of PH with right ventricular (RV) failure. Furthermore, we present how to characterize it hemodynamically by invasive measurements of RV and pulmonary arterial (PA) pressures. Animals subjected to this model display severe pulmonary vascular remodeling and RV dysfunction. In this model, rats undergo a single subcutaneous injection of Sugen (SU5416, a vascular endothelial growth factor inhibitor) and are immediately exposed to chronic hypoxia in a hypoxia chamber for 3–6 weeks. This Sugen/Hypoxia rat model resembles Group 1 PH.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages163-172
Number of pages10
DOIs
StatePublished - 2024
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2803
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cardiac hemodynamics
  • Pulmonary hypertension
  • Pulmonary vascular remodeling
  • Pulmonary vascular remodeling
  • Rat model
  • Right heart failure
  • Sugen/Hypoxia

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