TY - CHAP
T1 - The Sugen/Hypoxia Rat Model for Pulmonary Hypertension and Right Heart Failure
AU - Bikou, Olympia
AU - Sassi, Yassine
N1 - Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
PY - 2024
Y1 - 2024
N2 - Pulmonary hypertension (PH) is a devastating disease, characterized by complex remodeling of the pulmonary vasculature. PH is classified into five groups based on different etiology, pathology, as well as therapy and prognosis. Animal models are essential for the study of underlying mechanisms, pathophysiology, and preclinical testing of new therapies for PH. The complexity of the disease with different clinical entities dictates the necessity for more than one animal model to resemble PH, as a single model cannot imitate the broad spectrum of human PH. Here we describe a detailed protocol for creating a rat model of PH with right ventricular (RV) failure. Furthermore, we present how to characterize it hemodynamically by invasive measurements of RV and pulmonary arterial (PA) pressures. Animals subjected to this model display severe pulmonary vascular remodeling and RV dysfunction. In this model, rats undergo a single subcutaneous injection of Sugen (SU5416, a vascular endothelial growth factor inhibitor) and are immediately exposed to chronic hypoxia in a hypoxia chamber for 3–6 weeks. This Sugen/Hypoxia rat model resembles Group 1 PH.
AB - Pulmonary hypertension (PH) is a devastating disease, characterized by complex remodeling of the pulmonary vasculature. PH is classified into five groups based on different etiology, pathology, as well as therapy and prognosis. Animal models are essential for the study of underlying mechanisms, pathophysiology, and preclinical testing of new therapies for PH. The complexity of the disease with different clinical entities dictates the necessity for more than one animal model to resemble PH, as a single model cannot imitate the broad spectrum of human PH. Here we describe a detailed protocol for creating a rat model of PH with right ventricular (RV) failure. Furthermore, we present how to characterize it hemodynamically by invasive measurements of RV and pulmonary arterial (PA) pressures. Animals subjected to this model display severe pulmonary vascular remodeling and RV dysfunction. In this model, rats undergo a single subcutaneous injection of Sugen (SU5416, a vascular endothelial growth factor inhibitor) and are immediately exposed to chronic hypoxia in a hypoxia chamber for 3–6 weeks. This Sugen/Hypoxia rat model resembles Group 1 PH.
KW - Cardiac hemodynamics
KW - Pulmonary hypertension
KW - Pulmonary vascular remodeling
KW - Pulmonary vascular remodeling
KW - Rat model
KW - Right heart failure
KW - Sugen/Hypoxia
UR - http://www.scopus.com/inward/record.url?scp=85191618950&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-3846-0_12
DO - 10.1007/978-1-0716-3846-0_12
M3 - Chapter
C2 - 38676892
AN - SCOPUS:85191618950
T3 - Methods in Molecular Biology
SP - 163
EP - 172
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -