The structure of the nucleoprotein of Influenza D shows that all Orthomyxoviridae nucleoproteins have a similar NPCORE, with or without a NPTAIL for nuclear transport

Amélie Donchet; Justine Oliva; Alice Labaronne; Laura Tengo; Myriam Miloudi; Francine C.A. Gerard; Caroline Mas; Guy Schoehn; Rob W.H. Ruigrok; Mariette Ducatez; Thibaut Crépin

doi:10.1038/s41598-018-37306-y

The structure of the nucleoprotein of Influenza D shows that all Orthomyxoviridae nucleoproteins have a similar NP_CORE, with or without a NP_TAIL for nuclear transport

Amélie Donchet
, Justine Oliva
, Alice Labaronne
, Laura Tengo
, Myriam Miloudi
, Francine C.A. Gerard
, Caroline Mas
, Guy Schoehn
, Rob W.H. Ruigrok
, Mariette Ducatez
, Thibaut Crépin

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

This paper focuses on the nucleoprotein (NP) of the newly identified member of the Orthomyxoviridae family, Influenza D virus. To date several X-ray structures of NP of Influenza A (A/NP) and B (B/NP) viruses and of infectious salmon anemia (ISA/NP) virus have been solved. Here we purified, characterized and solved the X-ray structure of the tetrameric D/NP at 2.4 Å resolution. The crystal structure of its core is similar to NP of other Influenza viruses. However, unlike A/NP and B/NP which possess a flexible amino-terminal tail containing nuclear localization signals (NLS) for their nuclear import, D/NP possesses a carboxy-terminal tail (D/NP_TAIL). We show that D/NP_TAIL harbors a bipartite NLS and designed C-terminal truncated mutants to demonstrate the role of D/NP_TAIL for nuclear transport.

Original language	English
Article number	600
Journal	Scientific Reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-37306-y
State	Published - 1 Dec 2019
Externally published	Yes

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Donchet, A., Oliva, J., Labaronne, A., Tengo, L., Miloudi, M., C.A. Gerard, F., Mas, C., Schoehn, G., W.H. Ruigrok, R., Ducatez, M., & Crépin, T. (2019). The structure of the nucleoprotein of Influenza D shows that all Orthomyxoviridae nucleoproteins have a similar NP_CORE, with or without a NP_TAIL for nuclear transport. Scientific Reports, 9(1), Article 600. https://doi.org/10.1038/s41598-018-37306-y

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title = "The structure of the nucleoprotein of Influenza D shows that all Orthomyxoviridae nucleoproteins have a similar NPCORE, with or without a NPTAIL for nuclear transport",

abstract = "This paper focuses on the nucleoprotein (NP) of the newly identified member of the Orthomyxoviridae family, Influenza D virus. To date several X-ray structures of NP of Influenza A (A/NP) and B (B/NP) viruses and of infectious salmon anemia (ISA/NP) virus have been solved. Here we purified, characterized and solved the X-ray structure of the tetrameric D/NP at 2.4 {\AA} resolution. The crystal structure of its core is similar to NP of other Influenza viruses. However, unlike A/NP and B/NP which possess a flexible amino-terminal tail containing nuclear localization signals (NLS) for their nuclear import, D/NP possesses a carboxy-terminal tail (D/NPTAIL). We show that D/NPTAIL harbors a bipartite NLS and designed C-terminal truncated mutants to demonstrate the role of D/NPTAIL for nuclear transport.",

author = "Am{\'e}lie Donchet and Justine Oliva and Alice Labaronne and Laura Tengo and Myriam Miloudi and \{C.A. Gerard\}, Francine and Caroline Mas and Guy Schoehn and \{W.H. Ruigrok\}, Rob and Mariette Ducatez and Thibaut Cr{\'e}pin",

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Donchet, A, Oliva, J, Labaronne, A, Tengo, L, Miloudi, M, C.A. Gerard, F, Mas, C, Schoehn, G, W.H. Ruigrok, R, Ducatez, M & Crépin, T 2019, 'The structure of the nucleoprotein of Influenza D shows that all Orthomyxoviridae nucleoproteins have a similar NP_CORE, with or without a NP_TAIL for nuclear transport', Scientific Reports, vol. 9, no. 1, 600. https://doi.org/10.1038/s41598-018-37306-y

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T1 - The structure of the nucleoprotein of Influenza D shows that all Orthomyxoviridae nucleoproteins have a similar NPCORE, with or without a NPTAIL for nuclear transport

AU - Donchet, Amélie

AU - Oliva, Justine

AU - Labaronne, Alice

AU - Tengo, Laura

AU - Miloudi, Myriam

AU - C.A. Gerard, Francine

AU - Mas, Caroline

AU - Schoehn, Guy

AU - W.H. Ruigrok, Rob

AU - Ducatez, Mariette

AU - Crépin, Thibaut

PY - 2019/12/1

Y1 - 2019/12/1

N2 - This paper focuses on the nucleoprotein (NP) of the newly identified member of the Orthomyxoviridae family, Influenza D virus. To date several X-ray structures of NP of Influenza A (A/NP) and B (B/NP) viruses and of infectious salmon anemia (ISA/NP) virus have been solved. Here we purified, characterized and solved the X-ray structure of the tetrameric D/NP at 2.4 Å resolution. The crystal structure of its core is similar to NP of other Influenza viruses. However, unlike A/NP and B/NP which possess a flexible amino-terminal tail containing nuclear localization signals (NLS) for their nuclear import, D/NP possesses a carboxy-terminal tail (D/NPTAIL). We show that D/NPTAIL harbors a bipartite NLS and designed C-terminal truncated mutants to demonstrate the role of D/NPTAIL for nuclear transport.

AB - This paper focuses on the nucleoprotein (NP) of the newly identified member of the Orthomyxoviridae family, Influenza D virus. To date several X-ray structures of NP of Influenza A (A/NP) and B (B/NP) viruses and of infectious salmon anemia (ISA/NP) virus have been solved. Here we purified, characterized and solved the X-ray structure of the tetrameric D/NP at 2.4 Å resolution. The crystal structure of its core is similar to NP of other Influenza viruses. However, unlike A/NP and B/NP which possess a flexible amino-terminal tail containing nuclear localization signals (NLS) for their nuclear import, D/NP possesses a carboxy-terminal tail (D/NPTAIL). We show that D/NPTAIL harbors a bipartite NLS and designed C-terminal truncated mutants to demonstrate the role of D/NPTAIL for nuclear transport.

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The structure of the nucleoprotein of Influenza D shows that all Orthomyxoviridae nucleoproteins have a similar NPCORE, with or without a NPTAIL for nuclear transport

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The structure of the nucleoprotein of Influenza D shows that all Orthomyxoviridae nucleoproteins have a similar NP_CORE, with or without a NP_TAIL for nuclear transport