The RNA-methyltransferase misu (NSun2) poises epidermal stem cells to differentiate

Sandra Blanco; Agata Kurowski; Jennifer Nichols; Fiona M. Watt; Salvador Aznar Benitah; Michaela Frye

doi:10.1371/journal.pgen.1002403

The RNA-methyltransferase misu (NSun2) poises epidermal stem cells to differentiate

Sandra Blanco, Agata Kurowski, Jennifer Nichols, Fiona M. Watt, Salvador Aznar Benitah, Michaela Frye

Research output: Contribution to journal › Article › peer-review

158 Scopus citations

Abstract

Homeostasis of most adult tissues is maintained by balancing stem cell self-renewal and differentiation, but whether post-transcriptional mechanisms can regulate this process is unknown. Here, we identify that an RNA methyltransferase (Misu/Nsun2) is required to balance stem cell self-renewal and differentiation in skin. In the epidermis, this methyltransferase is found in a defined sub-population of hair follicle stem cells poised to undergo lineage commitment, and its depletion results in enhanced quiescence and aberrant stem cell differentiation. Our results reveal that post-transcriptional RNA methylation can play a previously unappreciated role in controlling stem cell fate.

Original language	English
Article number	e1002403
Journal	PLoS Genetics
Volume	7
Issue number	12
DOIs	https://doi.org/10.1371/journal.pgen.1002403
State	Published - Dec 2011
Externally published	Yes

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abstract = "Homeostasis of most adult tissues is maintained by balancing stem cell self-renewal and differentiation, but whether post-transcriptional mechanisms can regulate this process is unknown. Here, we identify that an RNA methyltransferase (Misu/Nsun2) is required to balance stem cell self-renewal and differentiation in skin. In the epidermis, this methyltransferase is found in a defined sub-population of hair follicle stem cells poised to undergo lineage commitment, and its depletion results in enhanced quiescence and aberrant stem cell differentiation. Our results reveal that post-transcriptional RNA methylation can play a previously unappreciated role in controlling stem cell fate.",

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AU - Blanco, Sandra

AU - Kurowski, Agata

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AU - Benitah, Salvador Aznar

AU - Frye, Michaela

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AB - Homeostasis of most adult tissues is maintained by balancing stem cell self-renewal and differentiation, but whether post-transcriptional mechanisms can regulate this process is unknown. Here, we identify that an RNA methyltransferase (Misu/Nsun2) is required to balance stem cell self-renewal and differentiation in skin. In the epidermis, this methyltransferase is found in a defined sub-population of hair follicle stem cells poised to undergo lineage commitment, and its depletion results in enhanced quiescence and aberrant stem cell differentiation. Our results reveal that post-transcriptional RNA methylation can play a previously unappreciated role in controlling stem cell fate.

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The RNA-methyltransferase misu (NSun2) poises epidermal stem cells to differentiate

Abstract

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