The regulation of exogenous and endogenous class I MHC genes in a human tumor cell line, K562

Richard T. Maziarz; Steven J. Burakoff; Douglas V. Faller

doi:10.1016/0161-5890(90)90108-C

The regulation of exogenous and endogenous class I MHC genes in a human tumor cell line, K562

Richard T. Maziarz, Steven J. Burakoff, Douglas V. Faller

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9 Scopus citations

Abstract

Previous studies have implied the existence of a trans-dominant intracellular repressor able to down-regulate the expression of the entire family of class I MHC genes in the genome of the K.562 erythroleukemia cell line. This study demonstrates, however, that the transfection of human or murine class I genes into K562 cells leads to the cell surface expression of the transfected MHC gene product in all situations, even when several kilobases of 5′ flanking sequence were included in the transfected genes. The endogenous cellular class I MHC genes remained repressed in the transfected cells. These finclings suggest that repression of class I MHC gene expression in K562 may not be mediated predominantly by a trans-dominant repressor of MHC gene expression; rather, other more complex regulatory influences might exist.

Original language	English
Pages (from-to)	135-142
Number of pages	8
Journal	Molecular Immunology
Volume	27
Issue number	2
DOIs	https://doi.org/10.1016/0161-5890(90)90108-C
State	Published - Feb 1990
Externally published	Yes

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title = "The regulation of exogenous and endogenous class I MHC genes in a human tumor cell line, K562",

abstract = "Previous studies have implied the existence of a trans-dominant intracellular repressor able to down-regulate the expression of the entire family of class I MHC genes in the genome of the K.562 erythroleukemia cell line. This study demonstrates, however, that the transfection of human or murine class I genes into K562 cells leads to the cell surface expression of the transfected MHC gene product in all situations, even when several kilobases of 5′ flanking sequence were included in the transfected genes. The endogenous cellular class I MHC genes remained repressed in the transfected cells. These finclings suggest that repression of class I MHC gene expression in K562 may not be mediated predominantly by a trans-dominant repressor of MHC gene expression; rather, other more complex regulatory influences might exist.",

author = "Maziarz, {Richard T.} and Burakoff, {Steven J.} and Faller, {Douglas V.}",

note = "Funding Information: This work was supported by NIH gra nt A117258 and by a research grant from the American Cam :er Society. R.T.M. is a recipient of an American Heart As sociation Clinician Scientists Award. D.V.F. is the recipierr t of an American Cancer Society Senior Faculty Award.",

year = "1990",

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doi = "10.1016/0161-5890(90)90108-C",

language = "English",

volume = "27",

pages = "135--142",

journal = "Molecular Immunology",

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T1 - The regulation of exogenous and endogenous class I MHC genes in a human tumor cell line, K562

AU - Maziarz, Richard T.

AU - Burakoff, Steven J.

AU - Faller, Douglas V.

N1 - Funding Information: This work was supported by NIH gra nt A117258 and by a research grant from the American Cam :er Society. R.T.M. is a recipient of an American Heart As sociation Clinician Scientists Award. D.V.F. is the recipierr t of an American Cancer Society Senior Faculty Award.

PY - 1990/2

Y1 - 1990/2

N2 - Previous studies have implied the existence of a trans-dominant intracellular repressor able to down-regulate the expression of the entire family of class I MHC genes in the genome of the K.562 erythroleukemia cell line. This study demonstrates, however, that the transfection of human or murine class I genes into K562 cells leads to the cell surface expression of the transfected MHC gene product in all situations, even when several kilobases of 5′ flanking sequence were included in the transfected genes. The endogenous cellular class I MHC genes remained repressed in the transfected cells. These finclings suggest that repression of class I MHC gene expression in K562 may not be mediated predominantly by a trans-dominant repressor of MHC gene expression; rather, other more complex regulatory influences might exist.

AB - Previous studies have implied the existence of a trans-dominant intracellular repressor able to down-regulate the expression of the entire family of class I MHC genes in the genome of the K.562 erythroleukemia cell line. This study demonstrates, however, that the transfection of human or murine class I genes into K562 cells leads to the cell surface expression of the transfected MHC gene product in all situations, even when several kilobases of 5′ flanking sequence were included in the transfected genes. The endogenous cellular class I MHC genes remained repressed in the transfected cells. These finclings suggest that repression of class I MHC gene expression in K562 may not be mediated predominantly by a trans-dominant repressor of MHC gene expression; rather, other more complex regulatory influences might exist.

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The regulation of exogenous and endogenous class I MHC genes in a human tumor cell line, K562

Abstract

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