The purification and properties of ribosomes from the thymus nucleus

A. O. Pogo; B. G.T. Pogo; V. C. Littau; V. G. Allfrey; A. E. Mirsky; M. G. Hamilton

doi:10.1016/0006-3002(62)90898-3

The purification and properties of ribosomes from the thymus nucleus

A. O. Pogo
, B. G.T. Pogo
, V. C. Littau
, V. G. Allfrey
, A. E. Mirsky
, M. G. Hamilton

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Methods are described for the isolation and purification of ribonucleoprotein particles (ribosomes) from isolated calf-thymus nuclei. About half of the total ribonucleic acid of the nucleus can be recovered in the purified ribosome fraction. Nuclear ribosomes can be purified by centrifugation in a sucrose density-gradient. The ribosome peak contains about 36% RNA. Treatment of the peak material with deoxycholate removes much of the protein. The resulting ribosomes have a sedimentation coefficient of 78 S and about 60% of their mass is RNA. The average base composition of this ribosomal RNA is given and compared with that of other nuclear RNA fractions. The changes in state of the nuclear 78-S ribosome with changing pH, ionic strength, and Mg²⁺ concentration are described. Electron microscopy of isolated ribosomes after shadow-casting with chromium indicates that the 78-S ribosomes are about 200 Å in width.

Original language	English
Pages (from-to)	849-864
Number of pages	16
Journal	BBA - Biochimica et Biophysica Acta
Volume	55
Issue number	6
DOIs	https://doi.org/10.1016/0006-3002(62)90898-3
State	Published - 11 Jun 1962
Externally published	Yes

Access to Document

10.1016/0006-3002(62)90898-3

Cite this

@article{252a453e351642d6ab24b09ae1001361,

title = "The purification and properties of ribosomes from the thymus nucleus",

abstract = "Methods are described for the isolation and purification of ribonucleoprotein particles (ribosomes) from isolated calf-thymus nuclei. About half of the total ribonucleic acid of the nucleus can be recovered in the purified ribosome fraction. Nuclear ribosomes can be purified by centrifugation in a sucrose density-gradient. The ribosome peak contains about 36\% RNA. Treatment of the peak material with deoxycholate removes much of the protein. The resulting ribosomes have a sedimentation coefficient of 78 S and about 60\% of their mass is RNA. The average base composition of this ribosomal RNA is given and compared with that of other nuclear RNA fractions. The changes in state of the nuclear 78-S ribosome with changing pH, ionic strength, and Mg2+ concentration are described. Electron microscopy of isolated ribosomes after shadow-casting with chromium indicates that the 78-S ribosomes are about 200 {\AA} in width.",

author = "Pogo, \{A. O.\} and Pogo, \{B. G.T.\} and Littau, \{V. C.\} and Allfrey, \{V. G.\} and Mirsky, \{A. E.\} and Hamilton, \{M. G.\}",

note = "Funding Information: The authors wish to thank Dr. M. L. PETERMANN of the Sloan-Kettering Institute for her advice and for many helpful discussions. This research was supported in part by a Grant (RG 4919) from the U.S. Pubhc Health Service. The first Author is a Fellow of the Guggenheim Foundation, and B. G. T. P. a Fellow of the Consejo Nacional de Investigaciones Cientlficas y T6cnicas, of the Argentine Republic. V. C. L. and M. G. H. were supported by the United States Atomic Energy Commission, the latter under their Contract AT(3o-I)-9io.",

year = "1962",

month = jun,

day = "11",

doi = "10.1016/0006-3002(62)90898-3",

language = "English",

volume = "55",

pages = "849--864",

journal = "BBA - Biochimica et Biophysica Acta",

issn = "0006-3002",

number = "6",

}

TY - JOUR

T1 - The purification and properties of ribosomes from the thymus nucleus

AU - Pogo, A. O.

AU - Pogo, B. G.T.

AU - Littau, V. C.

AU - Allfrey, V. G.

AU - Mirsky, A. E.

AU - Hamilton, M. G.

N1 - Funding Information: The authors wish to thank Dr. M. L. PETERMANN of the Sloan-Kettering Institute for her advice and for many helpful discussions. This research was supported in part by a Grant (RG 4919) from the U.S. Pubhc Health Service. The first Author is a Fellow of the Guggenheim Foundation, and B. G. T. P. a Fellow of the Consejo Nacional de Investigaciones Cientlficas y T6cnicas, of the Argentine Republic. V. C. L. and M. G. H. were supported by the United States Atomic Energy Commission, the latter under their Contract AT(3o-I)-9io.

PY - 1962/6/11

Y1 - 1962/6/11

N2 - Methods are described for the isolation and purification of ribonucleoprotein particles (ribosomes) from isolated calf-thymus nuclei. About half of the total ribonucleic acid of the nucleus can be recovered in the purified ribosome fraction. Nuclear ribosomes can be purified by centrifugation in a sucrose density-gradient. The ribosome peak contains about 36% RNA. Treatment of the peak material with deoxycholate removes much of the protein. The resulting ribosomes have a sedimentation coefficient of 78 S and about 60% of their mass is RNA. The average base composition of this ribosomal RNA is given and compared with that of other nuclear RNA fractions. The changes in state of the nuclear 78-S ribosome with changing pH, ionic strength, and Mg2+ concentration are described. Electron microscopy of isolated ribosomes after shadow-casting with chromium indicates that the 78-S ribosomes are about 200 Å in width.

AB - Methods are described for the isolation and purification of ribonucleoprotein particles (ribosomes) from isolated calf-thymus nuclei. About half of the total ribonucleic acid of the nucleus can be recovered in the purified ribosome fraction. Nuclear ribosomes can be purified by centrifugation in a sucrose density-gradient. The ribosome peak contains about 36% RNA. Treatment of the peak material with deoxycholate removes much of the protein. The resulting ribosomes have a sedimentation coefficient of 78 S and about 60% of their mass is RNA. The average base composition of this ribosomal RNA is given and compared with that of other nuclear RNA fractions. The changes in state of the nuclear 78-S ribosome with changing pH, ionic strength, and Mg2+ concentration are described. Electron microscopy of isolated ribosomes after shadow-casting with chromium indicates that the 78-S ribosomes are about 200 Å in width.

UR - https://www.scopus.com/pages/publications/50549178979

U2 - 10.1016/0006-3002(62)90898-3

DO - 10.1016/0006-3002(62)90898-3

M3 - Article

C2 - 14487392

AN - SCOPUS:50549178979

SN - 0006-3002

VL - 55

SP - 849

EP - 864

JO - BBA - Biochimica et Biophysica Acta

JF - BBA - Biochimica et Biophysica Acta

IS - 6

ER -

The purification and properties of ribosomes from the thymus nucleus

Abstract

Access to Document

Fingerprint

Cite this