The purification and properties of ribosomes from the thymus nucleus

A. O. Pogo, B. G.T. Pogo, V. C. Littau, V. G. Allfrey, A. E. Mirsky, M. G. Hamilton

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Methods are described for the isolation and purification of ribonucleoprotein particles (ribosomes) from isolated calf-thymus nuclei. About half of the total ribonucleic acid of the nucleus can be recovered in the purified ribosome fraction. Nuclear ribosomes can be purified by centrifugation in a sucrose density-gradient. The ribosome peak contains about 36% RNA. Treatment of the peak material with deoxycholate removes much of the protein. The resulting ribosomes have a sedimentation coefficient of 78 S and about 60% of their mass is RNA. The average base composition of this ribosomal RNA is given and compared with that of other nuclear RNA fractions. The changes in state of the nuclear 78-S ribosome with changing pH, ionic strength, and Mg2+ concentration are described. Electron microscopy of isolated ribosomes after shadow-casting with chromium indicates that the 78-S ribosomes are about 200 Å in width.

Original languageEnglish
Pages (from-to)849-864
Number of pages16
JournalBBA - Biochimica et Biophysica Acta
Volume55
Issue number6
DOIs
StatePublished - 11 Jun 1962
Externally publishedYes

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