The PLC/PRF/5 human hepatoma cell line. II. Chromosomal assignment of hepatitis B virus integration sites

A. M. Bowcock; M. R. Pinto; E. Bey; J. M. Kuyl; G. M. Dusheiko; R. Bernstein

doi:10.1016/0165-4608(85)90034-2

The PLC/PRF/5 human hepatoma cell line. II. Chromosomal assignment of hepatitis B virus integration sites

A. M. Bowcock
, M. R. Pinto
, E. Bey
, J. M. Kuyl
, G. M. Dusheiko
, R. Bernstein

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

The chromosomal sites at which hepatitis B virus (HBV) DNA is integrated into the genome of the hepatocellular carcinoma (HCC) cell line, PLC/PRF/5 were investigated in an attempt to understand the mechanisms by which hepatitis B virus may induce malignant transformation. In situ hybridization of an HBV DNA probe to metaphase chromosomes of the PLC/PRF/5 cell line, followed by statistical analysis, identified three integration sites; these were 15q22-q23, 11q22, and 18q12. In particular, hybridization to chromosome #15, which is present in four copies in complete metaphases of this cell line, was highly significant (p≪0.0005).

Original language	English
Pages (from-to)	19-26
Number of pages	8
Journal	Cancer Genetics and Cytogenetics
Volume	18
Issue number	1
DOIs	https://doi.org/10.1016/0165-4608(85)90034-2
State	Published - Sep 1985
Externally published	Yes

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title = "The PLC/PRF/5 human hepatoma cell line. II. Chromosomal assignment of hepatitis B virus integration sites",

abstract = "The chromosomal sites at which hepatitis B virus (HBV) DNA is integrated into the genome of the hepatocellular carcinoma (HCC) cell line, PLC/PRF/5 were investigated in an attempt to understand the mechanisms by which hepatitis B virus may induce malignant transformation. In situ hybridization of an HBV DNA probe to metaphase chromosomes of the PLC/PRF/5 cell line, followed by statistical analysis, identified three integration sites; these were 15q22-q23, 11q22, and 18q12. In particular, hybridization to chromosome \#15, which is present in four copies in complete metaphases of this cell line, was highly significant (p≪0.0005).",

author = "Bowcock, \{A. M.\} and Pinto, \{M. R.\} and E. Bey and Kuyl, \{J. M.\} and Dusheiko, \{G. M.\} and R. Bernstein",

year = "1985",

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doi = "10.1016/0165-4608(85)90034-2",

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T1 - The PLC/PRF/5 human hepatoma cell line. II. Chromosomal assignment of hepatitis B virus integration sites

AU - Bowcock, A. M.

AU - Pinto, M. R.

AU - Bey, E.

AU - Kuyl, J. M.

AU - Dusheiko, G. M.

AU - Bernstein, R.

PY - 1985/9

Y1 - 1985/9

N2 - The chromosomal sites at which hepatitis B virus (HBV) DNA is integrated into the genome of the hepatocellular carcinoma (HCC) cell line, PLC/PRF/5 were investigated in an attempt to understand the mechanisms by which hepatitis B virus may induce malignant transformation. In situ hybridization of an HBV DNA probe to metaphase chromosomes of the PLC/PRF/5 cell line, followed by statistical analysis, identified three integration sites; these were 15q22-q23, 11q22, and 18q12. In particular, hybridization to chromosome #15, which is present in four copies in complete metaphases of this cell line, was highly significant (p≪0.0005).

AB - The chromosomal sites at which hepatitis B virus (HBV) DNA is integrated into the genome of the hepatocellular carcinoma (HCC) cell line, PLC/PRF/5 were investigated in an attempt to understand the mechanisms by which hepatitis B virus may induce malignant transformation. In situ hybridization of an HBV DNA probe to metaphase chromosomes of the PLC/PRF/5 cell line, followed by statistical analysis, identified three integration sites; these were 15q22-q23, 11q22, and 18q12. In particular, hybridization to chromosome #15, which is present in four copies in complete metaphases of this cell line, was highly significant (p≪0.0005).

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JO - Cancer Genetics and Cytogenetics

JF - Cancer Genetics and Cytogenetics

IS - 1

ER -

The PLC/PRF/5 human hepatoma cell line. II. Chromosomal assignment of hepatitis B virus integration sites

Abstract

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