The PLC/PRF/5 human hepatoma cell line. II. Chromosomal assignment of hepatitis B virus integration sites

A. M. Bowcock, M. R. Pinto, E. Bey, J. M. Kuyl, G. M. Dusheiko, R. Bernstein

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The chromosomal sites at which hepatitis B virus (HBV) DNA is integrated into the genome of the hepatocellular carcinoma (HCC) cell line, PLC/PRF/5 were investigated in an attempt to understand the mechanisms by which hepatitis B virus may induce malignant transformation. In situ hybridization of an HBV DNA probe to metaphase chromosomes of the PLC/PRF/5 cell line, followed by statistical analysis, identified three integration sites; these were 15q22-q23, 11q22, and 18q12. In particular, hybridization to chromosome #15, which is present in four copies in complete metaphases of this cell line, was highly significant (p≪0.0005).

Original languageEnglish
Pages (from-to)19-26
Number of pages8
JournalCancer Genetics and Cytogenetics
Volume18
Issue number1
DOIs
StatePublished - Sep 1985
Externally publishedYes

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