The nutritional supplement Active Hexose Correlated Compound (AHCC) has direct immunomodulatory actions on intestinal epithelial cells and macrophages involving TLR/MyD88 and NF-κB/MAPK activation

Abdelali Daddaoua, Enrique Martínez-Plata, Mercedes Ortega-González, Borja Ocón, Carlos J. Aranda, Antonio Zarzuelo, María D. Suárez, Fermín Sánchez De Medina, Olga Martínez-Augustin

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Active Hexose Correlated Compound (AHCC) is an immunostimulatory nutritional supplement. AHCC effects and mechanism of action on intestinal epithelial cells or monocytes are poorly described. AHCC was added to the culture medium of intestinal epithelial cells (IEC18 and HT29 cells) and monocytes (THP-1 cells) and assessed the secretion of proinflammatory cytokines by ELISA. Inhibitors of NFjB and MAPKs were used to study signal transduction pathways while TLR4 and MyD88 were silenced in IEC18 cells using shRNA. It was found that AHCC induced GROα and MCP1 secretion in IEC18 and IL-8 in HT29 cells. These effects depended on NFjB activation, and partly on MAPKs activation and on the presence of MyD88 and TLR4. In THP-1 cells AHCC evoked IL-8, IL-1β and TNF-α secretion. The induction of IL-8 depended on JNK and NFjB activation. Therefore, AHCC exerts immunostimulatory effects on intestinal epithelial cells and monocytes involving TLR4/MyD88 and NFjB/MAPK signal transduction pathways.

Original languageEnglish
Pages (from-to)1288-1295
Number of pages8
JournalFood Chemistry
Volume136
Issue number3-4
DOIs
StatePublished - 15 Feb 2013
Externally publishedYes

Keywords

  • AHCC
  • Enterocyte
  • Macrophage immunomodulatory
  • NFκB
  • TLR4

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