TY - JOUR
T1 - The molecular characterization of the fetal stem cell marker AA4
AU - Petrenko, Oleksi
AU - Beavis, Andrew
AU - Klaine, Michele
AU - Kittappa, Raja
AU - Godin, Isabelle
AU - Lemischka, Ihor R.
N1 - Funding Information:
Correspondence should be addressed to I. R. L. We would like to thank Dr. Kateri Moore and Dr. Luc Pardanaud for helpful discussions, Dr. Jan Visser for enriched fractions of BM stem cells, Dr. Gordon Keller for differentiated ES cells, Dr. Garry Nolan for the retrovirus packaging cell line Phoenix E and plasmid LZRSpBMN-Z, and Dr. Schickwann Tsai for the EML C1 cell line. This work was supported in part by the Centre National de la Recherche Scientifique and a grant (n9455) from the Association pour la Recherche sur le Cancer (to I. G. and M. K.) and National Institutes of Health grants R01 DK 42989 and R01 CA 45339 (to I. R. L.).
PY - 1999/6
Y1 - 1999/6
N2 - We have identified and characterized the stem cell antigen AA4. This molecule is a type I transmembrane protein whose overall structure suggests a role in cell adhesion. During fetal ontogeny (days 9-14 of development), AA4 is expressed in three major cell types: vascular endothelial cells, aorta- associated hematopoietic clusters, and primitive fetal liver hematopoietic progenitors. In the adult, AA4 is abundant in lung, heart, and whole bone marrow. In the adult hematopoietic compartment, aa4 transcripts are present in bone marrow CD34(-/lo)Lin-Sca-1+c-Kit+ and CD34(hi) Lin-Sca-1+c-Kit+ stem and progenitor cell subsets. Our observations suggest that AA4 plays a role in cell-cell interactions during hematopoietic and vascular development.
AB - We have identified and characterized the stem cell antigen AA4. This molecule is a type I transmembrane protein whose overall structure suggests a role in cell adhesion. During fetal ontogeny (days 9-14 of development), AA4 is expressed in three major cell types: vascular endothelial cells, aorta- associated hematopoietic clusters, and primitive fetal liver hematopoietic progenitors. In the adult, AA4 is abundant in lung, heart, and whole bone marrow. In the adult hematopoietic compartment, aa4 transcripts are present in bone marrow CD34(-/lo)Lin-Sca-1+c-Kit+ and CD34(hi) Lin-Sca-1+c-Kit+ stem and progenitor cell subsets. Our observations suggest that AA4 plays a role in cell-cell interactions during hematopoietic and vascular development.
UR - http://www.scopus.com/inward/record.url?scp=0033152381&partnerID=8YFLogxK
U2 - 10.1016/S1074-7613(00)80068-0
DO - 10.1016/S1074-7613(00)80068-0
M3 - Article
C2 - 10403644
AN - SCOPUS:0033152381
SN - 1074-7613
VL - 10
SP - 691
EP - 700
JO - Immunity
JF - Immunity
IS - 6
ER -