The metabolic sensor PASK is a histone 3 kinase that also regulates H3K4 methylation by associating with H3K4 MLL2 methyltransferase complex

Jimsheena V. Karakkat; Suneesh Kaimala; Sreejisha P. Sreedharan; Princy Jayaprakash; Ernest A. Adeghate; Suraiya A. Ansari; Ernesto Guccione; Eric P.K. Mensah-Brown; Bright Starling Emerald

doi:10.1093/nar/gkz786

The metabolic sensor PASK is a histone 3 kinase that also regulates H3K4 methylation by associating with H3K4 MLL2 methyltransferase complex

Jimsheena V. Karakkat, Suneesh Kaimala, Sreejisha P. Sreedharan, Princy Jayaprakash, Ernest A. Adeghate, Suraiya A. Ansari, Ernesto Guccione, Eric P.K. Mensah-Brown, Bright Starling Emerald

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12 Scopus citations

Abstract

The metabolic sensor Per-Arnt-Sim (Pas) domain-containing serine/threonine kinase (PASK) is expressed predominantly in the cytoplasm of different cell types, although a small percentage is also expressed in the nucleus. Herein, we show that the nuclear PASK associates with the mammalian H3K4 MLL2 methyltransferase complex and enhances H3K4 di- and tri-methylation. We also show that PASK is a histone kinase that phosphorylates H3 at T3, T6, S10 and T11. Taken together, these results suggest that PASK regulates two different H3 tail modifications involving H3K4 methylation and H3 phosphorylation. Using muscle satellite cell differentiation and functional analysis after loss or gain of Pask expression using the CRISPR/Cas9 system, we provide evidence that some of the regulatory functions of PASK during development and differentiation may occur through the regulation of these histone modifications.

Original language	English
Pages (from-to)	10086-10103
Number of pages	18
Journal	Nucleic Acids Research
Volume	47
Issue number	19
DOIs	https://doi.org/10.1093/nar/gkz786
State	Published - 4 Nov 2019
Externally published	Yes

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Karakkat, J. V., Kaimala, S., Sreedharan, S. P., Jayaprakash, P., Adeghate, E. A., Ansari, S. A., Guccione, E., Mensah-Brown, E. P. K., & Starling Emerald, B. (2019). The metabolic sensor PASK is a histone 3 kinase that also regulates H3K4 methylation by associating with H3K4 MLL2 methyltransferase complex. Nucleic Acids Research, 47(19), 10086-10103. https://doi.org/10.1093/nar/gkz786

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title = "The metabolic sensor PASK is a histone 3 kinase that also regulates H3K4 methylation by associating with H3K4 MLL2 methyltransferase complex",

abstract = "The metabolic sensor Per-Arnt-Sim (Pas) domain-containing serine/threonine kinase (PASK) is expressed predominantly in the cytoplasm of different cell types, although a small percentage is also expressed in the nucleus. Herein, we show that the nuclear PASK associates with the mammalian H3K4 MLL2 methyltransferase complex and enhances H3K4 di- and tri-methylation. We also show that PASK is a histone kinase that phosphorylates H3 at T3, T6, S10 and T11. Taken together, these results suggest that PASK regulates two different H3 tail modifications involving H3K4 methylation and H3 phosphorylation. Using muscle satellite cell differentiation and functional analysis after loss or gain of Pask expression using the CRISPR/Cas9 system, we provide evidence that some of the regulatory functions of PASK during development and differentiation may occur through the regulation of these histone modifications.",

author = "Karakkat, {Jimsheena V.} and Suneesh Kaimala and Sreedharan, {Sreejisha P.} and Princy Jayaprakash and Adeghate, {Ernest A.} and Ansari, {Suraiya A.} and Ernesto Guccione and Mensah-Brown, {Eric P.K.} and {Starling Emerald}, Bright",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2019..",

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doi = "10.1093/nar/gkz786",

language = "English",

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Karakkat, JV, Kaimala, S, Sreedharan, SP, Jayaprakash, P, Adeghate, EA, Ansari, SA, Guccione, E, Mensah-Brown, EPK & Starling Emerald, B 2019, 'The metabolic sensor PASK is a histone 3 kinase that also regulates H3K4 methylation by associating with H3K4 MLL2 methyltransferase complex', Nucleic Acids Research, vol. 47, no. 19, pp. 10086-10103. https://doi.org/10.1093/nar/gkz786

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T1 - The metabolic sensor PASK is a histone 3 kinase that also regulates H3K4 methylation by associating with H3K4 MLL2 methyltransferase complex

AU - Karakkat, Jimsheena V.

AU - Kaimala, Suneesh

AU - Sreedharan, Sreejisha P.

AU - Jayaprakash, Princy

AU - Adeghate, Ernest A.

AU - Ansari, Suraiya A.

AU - Guccione, Ernesto

AU - Mensah-Brown, Eric P.K.

AU - Starling Emerald, Bright

PY - 2019/11/4

Y1 - 2019/11/4

N2 - The metabolic sensor Per-Arnt-Sim (Pas) domain-containing serine/threonine kinase (PASK) is expressed predominantly in the cytoplasm of different cell types, although a small percentage is also expressed in the nucleus. Herein, we show that the nuclear PASK associates with the mammalian H3K4 MLL2 methyltransferase complex and enhances H3K4 di- and tri-methylation. We also show that PASK is a histone kinase that phosphorylates H3 at T3, T6, S10 and T11. Taken together, these results suggest that PASK regulates two different H3 tail modifications involving H3K4 methylation and H3 phosphorylation. Using muscle satellite cell differentiation and functional analysis after loss or gain of Pask expression using the CRISPR/Cas9 system, we provide evidence that some of the regulatory functions of PASK during development and differentiation may occur through the regulation of these histone modifications.

AB - The metabolic sensor Per-Arnt-Sim (Pas) domain-containing serine/threonine kinase (PASK) is expressed predominantly in the cytoplasm of different cell types, although a small percentage is also expressed in the nucleus. Herein, we show that the nuclear PASK associates with the mammalian H3K4 MLL2 methyltransferase complex and enhances H3K4 di- and tri-methylation. We also show that PASK is a histone kinase that phosphorylates H3 at T3, T6, S10 and T11. Taken together, these results suggest that PASK regulates two different H3 tail modifications involving H3K4 methylation and H3 phosphorylation. Using muscle satellite cell differentiation and functional analysis after loss or gain of Pask expression using the CRISPR/Cas9 system, we provide evidence that some of the regulatory functions of PASK during development and differentiation may occur through the regulation of these histone modifications.

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The metabolic sensor PASK is a histone 3 kinase that also regulates H3K4 methylation by associating with H3K4 MLL2 methyltransferase complex

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