The measurement of blood and plasma nitrite by chemiluminescence: Pitfalls and solutions

Mildred M. Pelletier; Petra Kleinbongard; Lorna Ringwood; Rania Hito; Christian J. Hunter; Alan N. Schechter; Mark T. Gladwin; André Dejam

doi:10.1016/j.freeradbiomed.2006.05.001

The measurement of blood and plasma nitrite by chemiluminescence: Pitfalls and solutions

Mildred M. Pelletier, Petra Kleinbongard, Lorna Ringwood, Rania Hito, Christian J. Hunter, Alan N. Schechter, Mark T. Gladwin, André Dejam

Research output: Contribution to journal › Review article › peer-review

145 Scopus citations

Abstract

There are a number of difficulties involved in the quantification of nitrite in biological systems. These difficulties result from oxidation of nitrite (within minutes) by heme proteins, such as hemoglobin, myoglobin, cytoglobin, and neuroglobin; its low levels in vivo; and its ubiquitous presence in laboratory buffers and glassware. The goal of this review is to present an assay suitable for the sensitive and specific measurement of intravascular nitrite in mammals using the chemiluminescence-based nitric oxide analyzer and to inform the reader on how to evade the pitfalls pertinent to nitrite determination in biological matrices.

Original language	English
Pages (from-to)	541-548
Number of pages	8
Journal	Free Radical Biology and Medicine
Volume	41
Issue number	4
DOIs	https://doi.org/10.1016/j.freeradbiomed.2006.05.001
State	Published - 15 Aug 2006
Externally published	Yes

Keywords

Chemiluminescence
Erythrocytes
Free radicals
Nitric oxide
Nitrite
Plasma

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10.1016/j.freeradbiomed.2006.05.001

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title = "The measurement of blood and plasma nitrite by chemiluminescence: Pitfalls and solutions",

abstract = "There are a number of difficulties involved in the quantification of nitrite in biological systems. These difficulties result from oxidation of nitrite (within minutes) by heme proteins, such as hemoglobin, myoglobin, cytoglobin, and neuroglobin; its low levels in vivo; and its ubiquitous presence in laboratory buffers and glassware. The goal of this review is to present an assay suitable for the sensitive and specific measurement of intravascular nitrite in mammals using the chemiluminescence-based nitric oxide analyzer and to inform the reader on how to evade the pitfalls pertinent to nitrite determination in biological matrices.",

keywords = "Chemiluminescence, Erythrocytes, Free radicals, Nitric oxide, Nitrite, Plasma",

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AU - Pelletier, Mildred M.

AU - Kleinbongard, Petra

AU - Ringwood, Lorna

AU - Hito, Rania

AU - Hunter, Christian J.

AU - Schechter, Alan N.

AU - Gladwin, Mark T.

AU - Dejam, André

PY - 2006/8/15

Y1 - 2006/8/15

N2 - There are a number of difficulties involved in the quantification of nitrite in biological systems. These difficulties result from oxidation of nitrite (within minutes) by heme proteins, such as hemoglobin, myoglobin, cytoglobin, and neuroglobin; its low levels in vivo; and its ubiquitous presence in laboratory buffers and glassware. The goal of this review is to present an assay suitable for the sensitive and specific measurement of intravascular nitrite in mammals using the chemiluminescence-based nitric oxide analyzer and to inform the reader on how to evade the pitfalls pertinent to nitrite determination in biological matrices.

AB - There are a number of difficulties involved in the quantification of nitrite in biological systems. These difficulties result from oxidation of nitrite (within minutes) by heme proteins, such as hemoglobin, myoglobin, cytoglobin, and neuroglobin; its low levels in vivo; and its ubiquitous presence in laboratory buffers and glassware. The goal of this review is to present an assay suitable for the sensitive and specific measurement of intravascular nitrite in mammals using the chemiluminescence-based nitric oxide analyzer and to inform the reader on how to evade the pitfalls pertinent to nitrite determination in biological matrices.

KW - Chemiluminescence

KW - Erythrocytes

KW - Free radicals

KW - Nitric oxide

KW - Nitrite

KW - Plasma

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DO - 10.1016/j.freeradbiomed.2006.05.001

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AN - SCOPUS:33746032936

SN - 0891-5849

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JF - Free Radical Biology and Medicine

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The measurement of blood and plasma nitrite by chemiluminescence: Pitfalls and solutions

Abstract

Keywords

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