The measurement of blood and plasma nitrite by chemiluminescence: Pitfalls and solutions

Mildred M. Pelletier, Petra Kleinbongard, Lorna Ringwood, Rania Hito, Christian J. Hunter, Alan N. Schechter, Mark T. Gladwin, André Dejam

Research output: Contribution to journalReview articlepeer-review

137 Scopus citations

Abstract

There are a number of difficulties involved in the quantification of nitrite in biological systems. These difficulties result from oxidation of nitrite (within minutes) by heme proteins, such as hemoglobin, myoglobin, cytoglobin, and neuroglobin; its low levels in vivo; and its ubiquitous presence in laboratory buffers and glassware. The goal of this review is to present an assay suitable for the sensitive and specific measurement of intravascular nitrite in mammals using the chemiluminescence-based nitric oxide analyzer and to inform the reader on how to evade the pitfalls pertinent to nitrite determination in biological matrices.

Original languageEnglish
Pages (from-to)541-548
Number of pages8
JournalFree Radical Biology and Medicine
Volume41
Issue number4
DOIs
StatePublished - 15 Aug 2006
Externally publishedYes

Keywords

  • Chemiluminescence
  • Erythrocytes
  • Free radicals
  • Nitric oxide
  • Nitrite
  • Plasma

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