The length of the flexible SNAREpin juxtamembrane region is a critical determinant of SNARE-dependent fusion

James A. McNew, Thomas Weber, Donald M. Engelman, Thomas H. Söllner, James E. Rothman

Research output: Contribution to journalArticlepeer-review

134 Scopus citations

Abstract

The topology of a SNARE complex bridging two docked vesicles could act as a mechanical couple to do work on the lipid bilayer resulting in fusion. To test this, we prepared a series of modified SNARE proteins and determined their effects on SNARE-dependent membrane fusion. When two helix-breaking proline residues are introduced into the juxtamembrane region of VAMP, there is little or no effect on fusion, and the same change in syntaxin 1A only reduced the extent and rate of fusion by half. The insertion of a flexible linker between the transmembrane domain and the conserved coiled-coil domain only moderately affected fusion; however, fusion efficiency systematically decreased with increasing length of the linker. Together, these results rule out a requirement for helical continuity and suggest that distance is a critical factor for membrane fusion.

Original languageEnglish
Pages (from-to)415-421
Number of pages7
JournalMolecular Cell
Volume4
Issue number3
DOIs
StatePublished - Sep 1999
Externally publishedYes

Fingerprint

Dive into the research topics of 'The length of the flexible SNAREpin juxtamembrane region is a critical determinant of SNARE-dependent fusion'. Together they form a unique fingerprint.

Cite this