The involvement of lethal giant larvae and Wnt signaling in bottle cell formation in Xenopus embryos

Sun Cheol Choi, Sergei Y. Sokol

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Lethal giant larvae (Lgl) plays a critical role in establishment of cell polarity in epithelial cells. While Frizzled/Dsh signaling has been implicated in the regulation of the localization and activity of Lgl, it remains unclear whether specific Wnt ligands are involved. Here we show that Wnt5a triggers the release of Lgl from the cell cortex into the cytoplasm with the concomitant decrease in Lgl stability. The observed changes in Lgl localization were independent of atypical PKC (aPKC), which is known to influence Lgl distribution. In ectodermal cells, both Wnt5a and Lgl triggered morphological and molecular changes characteristic of apical constriction, whereas depletion of their functions prevented endogenous and ectopic bottle cell formation. Furthermore, Lgl RNA partially rescued bottle cell formation in embryos injected with a dominant negative Wnt5a construct. These results suggest a molecular link between Wnt5a and Lgl that is essential for apical constriction during vertebrate gastrulation.

Original languageEnglish
Pages (from-to)68-75
Number of pages8
JournalDevelopmental Biology
Volume336
Issue number1
DOIs
StatePublished - 1 Dec 2009

Keywords

  • Apical constriction
  • Bottle cell
  • Lgl
  • Wnt5a
  • Xenopus

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