The intracellular sites of early replication and budding of SARS-coronavirus

Silke Stertz, Mike Reichelt, Martin Spiegel, Thomas Kuri, Luis Martínez-Sobrido, Adolfo García-Sastre, Friedemann Weber, Georg Kochs

Research output: Contribution to journalArticlepeer-review

277 Scopus citations

Abstract

In this study, we analyzed the replication and budding sites of severe acute respiratory syndrome coronavirus (SARS-CoV) at early time points of infection. We detected cytoplasmic accumulations containing the viral nucleocapsid protein, viral RNA and the non-structural protein nsp3. Using EM techniques, we found that these putative viral replication sites were associated with characteristic membrane tubules and double membrane vesicles that most probably originated from ER cisternae. In addition to its presence at the replication sites, N also accumulated in the Golgi region and colocalized with the viral spike protein. Immuno-EM revealed that budding occurred at membranes of the ERGIC (ER-Golgi intermediate compartment) and the Golgi region as early as 3 h post infection, demonstrating that SARS-CoV replicates surprisingly fast. Our data suggest that SARS-CoV establishes replication complexes at ER-derived membranes. Later on, viral nucleocapsids have to be transported to the budding sites in the Golgi region where the viral glycoproteins accumulate and particle formation occurs.

Original languageEnglish
Pages (from-to)304-315
Number of pages12
JournalVirology
Volume361
Issue number2
DOIs
StatePublished - 10 May 2007

Keywords

  • Budding
  • Double membrane vesicles
  • Replication
  • SARS-coronavirus

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