The intrabody targeting of hTERT attenuates the immortality of cancer cells

Xiangying Zhu; Nan Yang; Jianguo Cai; Guimei Yang; Shenghua Liang; Daming Ren

doi:10.2478/s11658-009-0032-2

The intrabody targeting of hTERT attenuates the immortality of cancer cells

Xiangying Zhu
, Nan Yang
, Jianguo Cai
, Guimei Yang
, Shenghua Liang
, Daming Ren

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells.

Original language	English
Pages (from-to)	32-45
Number of pages	14
Journal	Cellular and Molecular Biology Letters
Volume	15
Issue number	1
DOIs	https://doi.org/10.2478/s11658-009-0032-2
State	Published - Mar 2010
Externally published	Yes

Keywords

Anti-hTERT ScFv
Cancer
Immortality
Intrabody

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10.2478/s11658-009-0032-2

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title = "The intrabody targeting of hTERT attenuates the immortality of cancer cells",

abstract = "hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85\% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70\% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells.",

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T1 - The intrabody targeting of hTERT attenuates the immortality of cancer cells

AU - Zhu, Xiangying

AU - Yang, Nan

AU - Cai, Jianguo

AU - Yang, Guimei

AU - Liang, Shenghua

AU - Ren, Daming

PY - 2010/3

Y1 - 2010/3

N2 - hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells.

AB - hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells.

KW - Anti-hTERT ScFv

KW - Cancer

KW - Immortality

KW - Intrabody

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DO - 10.2478/s11658-009-0032-2

M3 - Article

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EP - 45

JO - Cellular and Molecular Biology Letters

JF - Cellular and Molecular Biology Letters

IS - 1

ER -

The intrabody targeting of hTERT attenuates the immortality of cancer cells

Abstract

Keywords

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