The interaction of brain insulin receptors with wheat germ agglutinin

Joshua Shemer; Derek LeRoith

doi:10.1016/0143-4179(87)90025-4

The interaction of brain insulin receptors with wheat germ agglutinin

Joshua Shemer
, Derek LeRoith

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Brain insulin receptors adsorb to and are recoverable from wheat germ agglutinin-agarose (WGA) columns. Similar results are obtained using dissuccinimidyl suberate (DSS)-crosslinked receptors or photoaffinity labeled receptors. WGA can be used for partial purification of brain insulin receptors provided the appropriate WGA preparation is chosen and the optimal ratio of receptor protein to lectin is achieved.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Neuropeptides
Volume	9
Issue number	1
DOIs	https://doi.org/10.1016/0143-4179(87)90025-4
State	Published - Jan 1987
Externally published	Yes

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title = "The interaction of brain insulin receptors with wheat germ agglutinin",

abstract = "Brain insulin receptors adsorb to and are recoverable from wheat germ agglutinin-agarose (WGA) columns. Similar results are obtained using dissuccinimidyl suberate (DSS)-crosslinked receptors or photoaffinity labeled receptors. WGA can be used for partial purification of brain insulin receptors provided the appropriate WGA preparation is chosen and the optimal ratio of receptor protein to lectin is achieved.",

author = "Joshua Shemer and Derek LeRoith",

note = "Funding Information: The authors wish to thank Dr. Kim Heidenreich for help in planning the experiments and interpreting the results. In addition, Drs. Jesse Roth, Simeon Taylor, Becky Pruss, Jose Hedo, William Lowe, Jr., and Maxine A. Lesniak helped in the preparation of the manuscript. This study was supported by an award from the Lita Annenberg Hazen fund awarded to Jesse Roth. We wish to thank Mrs. Violet Katz for her expert secretarial assistance.",

year = "1987",

month = jan,

doi = "10.1016/0143-4179(87)90025-4",

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T1 - The interaction of brain insulin receptors with wheat germ agglutinin

AU - Shemer, Joshua

AU - LeRoith, Derek

N1 - Funding Information: The authors wish to thank Dr. Kim Heidenreich for help in planning the experiments and interpreting the results. In addition, Drs. Jesse Roth, Simeon Taylor, Becky Pruss, Jose Hedo, William Lowe, Jr., and Maxine A. Lesniak helped in the preparation of the manuscript. This study was supported by an award from the Lita Annenberg Hazen fund awarded to Jesse Roth. We wish to thank Mrs. Violet Katz for her expert secretarial assistance.

PY - 1987/1

Y1 - 1987/1

N2 - Brain insulin receptors adsorb to and are recoverable from wheat germ agglutinin-agarose (WGA) columns. Similar results are obtained using dissuccinimidyl suberate (DSS)-crosslinked receptors or photoaffinity labeled receptors. WGA can be used for partial purification of brain insulin receptors provided the appropriate WGA preparation is chosen and the optimal ratio of receptor protein to lectin is achieved.

AB - Brain insulin receptors adsorb to and are recoverable from wheat germ agglutinin-agarose (WGA) columns. Similar results are obtained using dissuccinimidyl suberate (DSS)-crosslinked receptors or photoaffinity labeled receptors. WGA can be used for partial purification of brain insulin receptors provided the appropriate WGA preparation is chosen and the optimal ratio of receptor protein to lectin is achieved.

UR - https://www.scopus.com/pages/publications/0023141966

U2 - 10.1016/0143-4179(87)90025-4

DO - 10.1016/0143-4179(87)90025-4

M3 - Article

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SN - 0143-4179

VL - 9

SP - 1

EP - 8

JO - Neuropeptides

JF - Neuropeptides

IS - 1

ER -

The interaction of brain insulin receptors with wheat germ agglutinin

Abstract

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