The influenza c virus glycoprotein (HE) exhibits receptor-binding (hemagglutinin) and receptor-destroying (esterase) activities

Reinhard Vlasak; Mark Krystal; Mariana Nacht; Peter Palese

doi:10.1016/0042-6822(87)90013-4

The influenza c virus glycoprotein (HE) exhibits receptor-binding (hemagglutinin) and receptor-destroying (esterase) activities

Reinhard Vlasak, Mark Krystal, Mariana Nacht, Peter Palese

Research output: Contribution to journal › Article › peer-review

109 Scopus citations

Abstract

A cDNA copy of RNA segment 4 of influenza C/Cal/78 virus was cloned into an SV40 vector and expressed in CV1 cells. The gene product expressed from the SV40 recombinant virus was immunoprecipitated by monoclonal antibodies directed against the influenza C virus glycoprotein. Cells infected with the recombinant virus also exhibited C virus-specific hemagglutinin and O-acetylesterase activity. This suggests that the same C virus protein is associated with receptor-binding as well as receptor-destroying activity. The latter viral activity was measured using as substrates bovine submaxillary mucin or a low molecular weight compound p-nitrophenylacetate. In analogy to the parainfluenza virus HN protein, the influenza C virus glycoprotein was termed HE, because it possesses hemagglutinin and esterase (receptor-destroying) activity.

Original language	English
Pages (from-to)	419-425
Number of pages	7
Journal	Virology
Volume	160
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(87)90013-4
State	Published - Oct 1987

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title = "The influenza c virus glycoprotein (HE) exhibits receptor-binding (hemagglutinin) and receptor-destroying (esterase) activities",

abstract = "A cDNA copy of RNA segment 4 of influenza C/Cal/78 virus was cloned into an SV40 vector and expressed in CV1 cells. The gene product expressed from the SV40 recombinant virus was immunoprecipitated by monoclonal antibodies directed against the influenza C virus glycoprotein. Cells infected with the recombinant virus also exhibited C virus-specific hemagglutinin and O-acetylesterase activity. This suggests that the same C virus protein is associated with receptor-binding as well as receptor-destroying activity. The latter viral activity was measured using as substrates bovine submaxillary mucin or a low molecular weight compound p-nitrophenylacetate. In analogy to the parainfluenza virus HN protein, the influenza C virus glycoprotein was termed HE, because it possesses hemagglutinin and esterase (receptor-destroying) activity.",

author = "Reinhard Vlasak and Mark Krystal and Mariana Nacht and Peter Palese",

note = "Funding Information: We thank Drs . Tom Moran and Marc Monestier for supplying us with isotype-and light chain-specific monoclonal antibodies . This work was supported by Public Health Service Grants Al-11823 and Al-18908. R.V. Is recipient of a Max Kade postdoctoral fellowship. M .K . was supported by an Alexander and Alexandrine Sinsheimer Scholar award and by a Charles H . Revson fellowship .",

year = "1987",

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doi = "10.1016/0042-6822(87)90013-4",

language = "English",

volume = "160",

pages = "419--425",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

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T1 - The influenza c virus glycoprotein (HE) exhibits receptor-binding (hemagglutinin) and receptor-destroying (esterase) activities

AU - Vlasak, Reinhard

AU - Krystal, Mark

AU - Nacht, Mariana

AU - Palese, Peter

N1 - Funding Information: We thank Drs . Tom Moran and Marc Monestier for supplying us with isotype-and light chain-specific monoclonal antibodies . This work was supported by Public Health Service Grants Al-11823 and Al-18908. R.V. Is recipient of a Max Kade postdoctoral fellowship. M .K . was supported by an Alexander and Alexandrine Sinsheimer Scholar award and by a Charles H . Revson fellowship .

PY - 1987/10

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N2 - A cDNA copy of RNA segment 4 of influenza C/Cal/78 virus was cloned into an SV40 vector and expressed in CV1 cells. The gene product expressed from the SV40 recombinant virus was immunoprecipitated by monoclonal antibodies directed against the influenza C virus glycoprotein. Cells infected with the recombinant virus also exhibited C virus-specific hemagglutinin and O-acetylesterase activity. This suggests that the same C virus protein is associated with receptor-binding as well as receptor-destroying activity. The latter viral activity was measured using as substrates bovine submaxillary mucin or a low molecular weight compound p-nitrophenylacetate. In analogy to the parainfluenza virus HN protein, the influenza C virus glycoprotein was termed HE, because it possesses hemagglutinin and esterase (receptor-destroying) activity.

AB - A cDNA copy of RNA segment 4 of influenza C/Cal/78 virus was cloned into an SV40 vector and expressed in CV1 cells. The gene product expressed from the SV40 recombinant virus was immunoprecipitated by monoclonal antibodies directed against the influenza C virus glycoprotein. Cells infected with the recombinant virus also exhibited C virus-specific hemagglutinin and O-acetylesterase activity. This suggests that the same C virus protein is associated with receptor-binding as well as receptor-destroying activity. The latter viral activity was measured using as substrates bovine submaxillary mucin or a low molecular weight compound p-nitrophenylacetate. In analogy to the parainfluenza virus HN protein, the influenza C virus glycoprotein was termed HE, because it possesses hemagglutinin and esterase (receptor-destroying) activity.

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AN - SCOPUS:0023429281

SN - 0042-6822

VL - 160

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JO - Virology

JF - Virology

IS - 2

ER -

The influenza c virus glycoprotein (HE) exhibits receptor-binding (hemagglutinin) and receptor-destroying (esterase) activities

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