The induction of cytolytic T lymphocytes with syngeneic trinitrophenyl-coupled membranes

Recently we have demonstrated the induction of allogeneic murine cytolytic T lymphocytes (CTL)³ using purified plasma membranes rather than intact cells as stimulatory agents. In this report we extend the use of such subcellular preparations to study the requirements for hapten-specific syngeneic CTL induction. Membranes prepared from trinitrophenyl (TNP) coupled syngeneic tumor cells retain the ability to stimulate both a primary and secondary CTL response. The CTL that are generated are restricted in their lysis to target cells bearing the same H-2 antigens as those present on the TNP-coupled stimulating membranes.