The immunohistochemical demonstration of noradrenergic neurons in the rat brain: The use of homologous antiserum to dopamine-β-hydroxylase

Reinhard Grzanna; John H. Morrison; Joseph T. Coyle; Mark E. Molliver

doi:10.1016/0304-3940(77)90127-6

The immunohistochemical demonstration of noradrenergic neurons in the rat brain: The use of homologous antiserum to dopamine-β-hydroxylase

Reinhard Grzanna, John H. Morrison, Joseph T. Coyle, Mark E. Molliver

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Abstract

The immunohistochemical localization of dopamine-β-hydroxylase (DBH) in central noradrenergic neurons of the rat is greatly improved by the use of homologous antiserum (i.e. antibody against purified rat DBH). This antiserum, which has a several thousand-fold greater affinity for rat DBH than does heterologous antibody, is used at a 1:500 dilution with low concentrations of detergent. This method applied to paraformaldehyde-perfused tissue consistently yields highly sensitive and selective staining with excellent cytologic preservation of fine neuronal processes.

Original language	English
Pages (from-to)	127-134
Number of pages	8
Journal	Neuroscience Letters
Volume	4
Issue number	3-4
DOIs	https://doi.org/10.1016/0304-3940(77)90127-6
State	Published - Mar 1977
Externally published	Yes

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@article{338791f040d04241aebf134b30aabb56,

title = "The immunohistochemical demonstration of noradrenergic neurons in the rat brain: The use of homologous antiserum to dopamine-β-hydroxylase",

abstract = "The immunohistochemical localization of dopamine-β-hydroxylase (DBH) in central noradrenergic neurons of the rat is greatly improved by the use of homologous antiserum (i.e. antibody against purified rat DBH). This antiserum, which has a several thousand-fold greater affinity for rat DBH than does heterologous antibody, is used at a 1:500 dilution with low concentrations of detergent. This method applied to paraformaldehyde-perfused tissue consistently yields highly sensitive and selective staining with excellent cytologic preservation of fine neuronal processes.",

author = "Reinhard Grzanna and Morrison, \{John H.\} and Coyle, \{Joseph T.\} and Molliver, \{Mark E.\}",

note = "Funding Information: This re.arch was supported by U.S.P.H.S. Grants NS-08153, MH-26654, NS-10920, DA-00266, by a Basil O'Connor \textasciitilde{}ant from the March of Dimes, by United Cerebral Palsy Foundation Grant R 244-71, and by Deutsche For-schungsgemeinschaft Fellowship GR 504 to R.G. The authors wish to acknowledge the excellent technical assistance of Pamela Taylor.",

year = "1977",

month = mar,

doi = "10.1016/0304-3940(77)90127-6",

language = "English",

volume = "4",

pages = "127--134",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "3-4",

}

TY - JOUR

T1 - The immunohistochemical demonstration of noradrenergic neurons in the rat brain

T2 - The use of homologous antiserum to dopamine-β-hydroxylase

AU - Grzanna, Reinhard

AU - Morrison, John H.

AU - Coyle, Joseph T.

AU - Molliver, Mark E.

N1 - Funding Information: This re.arch was supported by U.S.P.H.S. Grants NS-08153, MH-26654, NS-10920, DA-00266, by a Basil O'Connor ~ant from the March of Dimes, by United Cerebral Palsy Foundation Grant R 244-71, and by Deutsche For-schungsgemeinschaft Fellowship GR 504 to R.G. The authors wish to acknowledge the excellent technical assistance of Pamela Taylor.

PY - 1977/3

Y1 - 1977/3

N2 - The immunohistochemical localization of dopamine-β-hydroxylase (DBH) in central noradrenergic neurons of the rat is greatly improved by the use of homologous antiserum (i.e. antibody against purified rat DBH). This antiserum, which has a several thousand-fold greater affinity for rat DBH than does heterologous antibody, is used at a 1:500 dilution with low concentrations of detergent. This method applied to paraformaldehyde-perfused tissue consistently yields highly sensitive and selective staining with excellent cytologic preservation of fine neuronal processes.

AB - The immunohistochemical localization of dopamine-β-hydroxylase (DBH) in central noradrenergic neurons of the rat is greatly improved by the use of homologous antiserum (i.e. antibody against purified rat DBH). This antiserum, which has a several thousand-fold greater affinity for rat DBH than does heterologous antibody, is used at a 1:500 dilution with low concentrations of detergent. This method applied to paraformaldehyde-perfused tissue consistently yields highly sensitive and selective staining with excellent cytologic preservation of fine neuronal processes.

UR - https://www.scopus.com/pages/publications/0017328793

U2 - 10.1016/0304-3940(77)90127-6

DO - 10.1016/0304-3940(77)90127-6

M3 - Article

AN - SCOPUS:0017328793

SN - 0304-3940

VL - 4

SP - 127

EP - 134

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 3-4

ER -

The immunohistochemical demonstration of noradrenergic neurons in the rat brain: The use of homologous antiserum to dopamine-β-hydroxylase

Abstract

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