TY - JOUR
T1 - The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology
AU - Schmitt-Engel, Christian
AU - Schultheis, Dorothea
AU - Schwirz, Jonas
AU - Ströhlein, Nadi
AU - Troelenberg, Nicole
AU - Majumdar, Upalparna
AU - Dao, Van Anh
AU - Grossmann, Daniela
AU - Richter, Tobias
AU - Tech, Maike
AU - Dönitz, Jürgen
AU - Gerischer, Lizzy
AU - Theis, Mirko
AU - Schild, Inga
AU - Trauner, Jochen
AU - Koniszewski, Nikolaus D.B.
AU - Küster, Elke
AU - Kittelmann, Sebastian
AU - Hu, Yonggang
AU - Lehmann, Sabrina
AU - Siemanowski, Janna
AU - Ulrich, Julia
AU - Panfilio, Kristen A.
AU - Schröder, Reinhard
AU - Morgenstern, Burkhard
AU - Stanke, Mario
AU - Buchhholz, Frank
AU - Frasch, Manfred
AU - Roth, Siegfried
AU - Wimmer, Ernst A.
AU - Schoppmeier, Michael
AU - Klingler, Martin
AU - Bucher, Gregor
N1 - Funding Information:
We thank Deutsche Forschungsgemeinschaft for funding this project (FOR1234 ‘iBeetle’) and the Georg-August-University Göttingen and the Friedrich-Alexander-University Erlangen-Nürnberg for financial and logistical support. We acknowledge support by the Open Access Publication Funds of the Georg-August-University Göttingen.
Publisher Copyright:
© 2015 Macmillan Publishers Limited. All rights reserved.
PY - 2015/7/28
Y1 - 2015/7/28
N2 - Genetic screens are powerful tools to identify the genes required for a given biological process. However, for technical reasons, comprehensive screens have been restricted to very few model organisms. Therefore, although deep sequencing is revealing the genes of ever more insect species, the functional studies predominantly focus on candidate genes previously identified in Drosophila, which is biasing research towards conserved gene functions. RNAi screens in other organisms promise to reduce this bias. Here we present the results of the iBeetle screen, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic development, physiology and cell biology. The utility of Tribolium as a screening platform is demonstrated by the identification of genes involved in insect epithelial adhesion. This work transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila.
AB - Genetic screens are powerful tools to identify the genes required for a given biological process. However, for technical reasons, comprehensive screens have been restricted to very few model organisms. Therefore, although deep sequencing is revealing the genes of ever more insect species, the functional studies predominantly focus on candidate genes previously identified in Drosophila, which is biasing research towards conserved gene functions. RNAi screens in other organisms promise to reduce this bias. Here we present the results of the iBeetle screen, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic development, physiology and cell biology. The utility of Tribolium as a screening platform is demonstrated by the identification of genes involved in insect epithelial adhesion. This work transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila.
UR - http://www.scopus.com/inward/record.url?scp=84938124727&partnerID=8YFLogxK
U2 - 10.1038/ncomms8822
DO - 10.1038/ncomms8822
M3 - Article
C2 - 26215380
AN - SCOPUS:84938124727
SN - 2041-1723
VL - 6
JO - Nature Communications
JF - Nature Communications
M1 - 7822
ER -