@article{01c6c7a4cb3b42eabb3c4a2951abe048,
title = "The good side of inflammation: Staphylococcus aureus proteins SpA and Sbi contribute to proper abscess formation and wound healing during skin and soft tissue infections",
abstract = "Staphylococcus aureus is the most prominent cause of skin and soft tissue infections (SSTI) worldwide. Mortality associated with invasive SSTI is a major threat to public health considering the incidence of antibiotic resistant isolates in particular methicillin resistant S. aureus both in the hospital (HA-MRSA) and in the community (CA-MRSA). To overcome the increasing difficulties in the clinical management of SSTI due to MRSA, new prophylactic and therapeutic approaches are urgently needed and a preventive vaccine would be welcome. The rational design of an anti-S. aureus vaccine requires a deep knowledge of the role that the different bacterial virulence factors play according to the type of infection. In the present study, using a set of isogenic deficient mutants and their complemented strains we determined that the staphylococcal surface proteins SpA and Sbi play an important role in the induction of inflammatory cytokines and chemokines in the skin during SSTI. SpA and Sbi initiate signaling cascades that lead to the early recruitment of neutrophils, modulate their lifespan in the skin milieu and contribute to proper abscess formation and bacterial eradication. Moreover, the expression of SpA and Sbi appear critical for skin repair and wound healing. Thus, these results indicate that SpA and Sbi can promote immune responses in the skin that are beneficial for the host and therefore, should not be neutralized with vaccine formulations designed to prevent SSTI.",
keywords = "Abscess, Sbi, Skin infections, SpA, Staphylococcus aureus",
author = "Gonzalez, {Cintia D.} and Camila Ledo and Eliana Cela and In{\'e}s Stella and Chunliang Xu and Ojeda, {Diego S.} and Frenette, {Paul S.} and G{\'o}mez, {Marisa I.}",
note = "Funding Information: The authors thank Monica Pomerantz, Lorena Medina, Colette Prophete and Paul Ciero for technical assistance as well as Dr. Timothy Foster, Dr. Joan Geoghegan and Dr. Alice Prince for providing bacterial strains. We also thank Ariel Billordo and Pl{\'a}cida Blas for technical assistance with flow cytometry and Vet. Marianela Lewicki for assistance with animal care. This work was supported in part by grants from the Agencia Nacional de Promoci{\'o}n Cient{\'i}fica y Tecnol{\'o}gica , Argentina ( ANPCYT PICT 2013-1233 and ANPCYT PICT 2016-2678 to MIG); the Secretar{\'i}a de Ciencia y T{\'e}cnica, Universidad de Buenos Aires , Argentina ( UBACyT 20020150100114BA to MIG), the Fulbright Commission and CONICET (to MIG), the National Institutes of Health ( R01 HL069438 (to PSF)). Intramural funding has been provided by the Fundaci{\'o}n Cient{\'i}fica Felipe Fiorellino . Funding Information: The authors thank Monica Pomerantz, Lorena Medina, Colette Prophete and Paul Ciero for technical assistance as well as Dr. Timothy Foster, Dr. Joan Geoghegan and Dr. Alice Prince for providing bacterial strains. We also thank Ariel Billordo and Pl?cida Blas for technical assistance with flow cytometry and Vet. Marianela Lewicki for assistance with animal care. This work was supported in part by grants from the Agencia Nacional de Promoci?n Cient?fica y Tecnol?gica, Argentina (ANPCYT PICT 2013-1233 and ANPCYT PICT 2016-2678 to MIG); the Secretar?a de Ciencia y T?cnica, Universidad de Buenos Aires, Argentina (UBACyT 20020150100114BA to MIG), the Fulbright Commission and CONICET (to MIG), the National Institutes of Health (R01 HL069438 (to PSF)). Intramural funding has been provided by the Fundaci?n Cient?fica Felipe Fiorellino. Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",
year = "2019",
month = oct,
day = "1",
doi = "10.1016/j.bbadis.2019.07.004",
language = "English",
volume = "1865",
pages = "2657--2670",
journal = "Biochimica et Biophysica Acta - Molecular Basis of Disease",
issn = "0925-4439",
publisher = "Elsevier",
number = "10",
}