The genetic program of hematopoietic stem cells

Robert L. Phillips; Robin E. Ernst; Brian Brunk; Natalia Ivanova; Mark A. Mahan; Julia K. Deanehan; Kateri A. Moore; G. Christian Overton; Ihor R. Lemischka

doi:10.1126/science.288.5471.1635

The genetic program of hematopoietic stem cells

Robert L. Phillips
, Robin E. Ernst
, Brian Brunk
, Natalia Ivanova
, Mark A. Mahan
, Julia K. Deanehan
, Kateri A. Moore
, G. Christian Overton
, Ihor R. Lemischka

Research output: Contribution to journal › Article › peer-review

373 Scopus citations

Abstract

Blood cell production originates from a rare population of multipotent, self-renewing stem cells. A genome-wide gene expression analysis was performed in order to define regulatory pathways in stem cells as well as their global genetic program. Subtracted complementary DNA libraries from highly purified murine fetal liver stem cells were analyzed with bioinformatic and array hybridization strategies. A large percentage of the several thousand gene products that have been characterized correspond to previously undescribed molecules with properties suggestive of regulatory functions. The complete data, available in a biological process-oriented database, represent the molecular phenotype of the hematopoietic stem cell.

Original language	English
Pages (from-to)	1635-1640
Number of pages	6
Journal	Science
Volume	288
Issue number	5471
DOIs	https://doi.org/10.1126/science.288.5471.1635
State	Published - 2 Jun 2000
Externally published	Yes

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title = "The genetic program of hematopoietic stem cells",

abstract = "Blood cell production originates from a rare population of multipotent, self-renewing stem cells. A genome-wide gene expression analysis was performed in order to define regulatory pathways in stem cells as well as their global genetic program. Subtracted complementary DNA libraries from highly purified murine fetal liver stem cells were analyzed with bioinformatic and array hybridization strategies. A large percentage of the several thousand gene products that have been characterized correspond to previously undescribed molecules with properties suggestive of regulatory functions. The complete data, available in a biological process-oriented database, represent the molecular phenotype of the hematopoietic stem cell.",

author = "Phillips, \{Robert L.\} and Ernst, \{Robin E.\} and Brian Brunk and Natalia Ivanova and Mahan, \{Mark A.\} and Deanehan, \{Julia K.\} and Moore, \{Kateri A.\} and Overton, \{G. Christian\} and Lemischka, \{Ihor R.\}",

year = "2000",

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doi = "10.1126/science.288.5471.1635",

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AU - Phillips, Robert L.

AU - Ernst, Robin E.

AU - Brunk, Brian

AU - Ivanova, Natalia

AU - Mahan, Mark A.

AU - Deanehan, Julia K.

AU - Moore, Kateri A.

AU - Overton, G. Christian

AU - Lemischka, Ihor R.

PY - 2000/6/2

Y1 - 2000/6/2

N2 - Blood cell production originates from a rare population of multipotent, self-renewing stem cells. A genome-wide gene expression analysis was performed in order to define regulatory pathways in stem cells as well as their global genetic program. Subtracted complementary DNA libraries from highly purified murine fetal liver stem cells were analyzed with bioinformatic and array hybridization strategies. A large percentage of the several thousand gene products that have been characterized correspond to previously undescribed molecules with properties suggestive of regulatory functions. The complete data, available in a biological process-oriented database, represent the molecular phenotype of the hematopoietic stem cell.

AB - Blood cell production originates from a rare population of multipotent, self-renewing stem cells. A genome-wide gene expression analysis was performed in order to define regulatory pathways in stem cells as well as their global genetic program. Subtracted complementary DNA libraries from highly purified murine fetal liver stem cells were analyzed with bioinformatic and array hybridization strategies. A large percentage of the several thousand gene products that have been characterized correspond to previously undescribed molecules with properties suggestive of regulatory functions. The complete data, available in a biological process-oriented database, represent the molecular phenotype of the hematopoietic stem cell.

UR - https://www.scopus.com/pages/publications/0034595656

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VL - 288

SP - 1635

EP - 1640

JO - Science

JF - Science

IS - 5471

ER -

The genetic program of hematopoietic stem cells

Abstract

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