The formation of bilirubin mono- and diglucuronide by rat liver microsomal fractions

Elias Halac; Margaret Dipiazza; Phillis Detwiler

doi:10.1016/0304-4165(72)90176-6

The formation of bilirubin mono- and diglucuronide by rat liver microsomal fractions

Elias Halac, Margaret Dipiazza, Phillis Detwiler

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The product of the in vitro reaction catalyzed by bilirubin UDPglucuronyl-transferase has been studied with an improved chromatographic procedure for the separation and measurement of the diazo pigments. The source of enzyme activity was total microsomes and the smooth and rough microsomal subfractions after dialysis against EDTA. Biliburin monoglucuronide was the only conjugate formed by smooth microsomes while total and rough microsomes formed bilirubin mono and diglucuronide. These findings are compatible with the presence in rough microsomes of a factor(s), not present in smooth microsomes, necessary for the formation of bilirubin diglucuronide. The activity of bilirubin UDPglucuronyltransferase in rough microsomes was higher than in the smooth fraction.

Original language	English
Pages (from-to)	544-553
Number of pages	10
Journal	Biochimica et Biophysica Acta - General Subjects
Volume	279
Issue number	3
DOIs	https://doi.org/10.1016/0304-4165(72)90176-6
State	Published - 25 Oct 1972
Externally published	Yes

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10.1016/0304-4165(72)90176-6

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title = "The formation of bilirubin mono- and diglucuronide by rat liver microsomal fractions",

abstract = "The product of the in vitro reaction catalyzed by bilirubin UDPglucuronyl-transferase has been studied with an improved chromatographic procedure for the separation and measurement of the diazo pigments. The source of enzyme activity was total microsomes and the smooth and rough microsomal subfractions after dialysis against EDTA. Biliburin monoglucuronide was the only conjugate formed by smooth microsomes while total and rough microsomes formed bilirubin mono and diglucuronide. These findings are compatible with the presence in rough microsomes of a factor(s), not present in smooth microsomes, necessary for the formation of bilirubin diglucuronide. The activity of bilirubin UDPglucuronyltransferase in rough microsomes was higher than in the smooth fraction.",

author = "Elias Halac and Margaret Dipiazza and Phillis Detwiler",

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AU - Halac, Elias

AU - Dipiazza, Margaret

AU - Detwiler, Phillis

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AB - The product of the in vitro reaction catalyzed by bilirubin UDPglucuronyl-transferase has been studied with an improved chromatographic procedure for the separation and measurement of the diazo pigments. The source of enzyme activity was total microsomes and the smooth and rough microsomal subfractions after dialysis against EDTA. Biliburin monoglucuronide was the only conjugate formed by smooth microsomes while total and rough microsomes formed bilirubin mono and diglucuronide. These findings are compatible with the presence in rough microsomes of a factor(s), not present in smooth microsomes, necessary for the formation of bilirubin diglucuronide. The activity of bilirubin UDPglucuronyltransferase in rough microsomes was higher than in the smooth fraction.

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The formation of bilirubin mono- and diglucuronide by rat liver microsomal fractions

Abstract

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