The epigenome of AML stem and progenitor cells

Jumpei Yamazaki, Marcos R. Estecio, Yue Lu, Hai Long, Gabriel G. Malouf, David Graber, Yujia Huo, Louis Ramagli, Shoudan Liang, Steven M. Kornblau, Jaroslav Jelinek, Jean Pierre J. Issa

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Acute myeloid leukemia (AML) is sustained by a population of cancer stem cells (CSC s or cancer-initiating cell). The mechanisms underlying switches from CSC s to non-CSC s in vivo remain to be understood. We address this issue in AML from the aspect of epigenetics using genome-wide screening for DNA methylation and selected histone modifications. We found no major differences in DNA methylation, especially in promoter CpG islands, between CSC s and non-CSC s. By contrast, we found thousands of genes that change H3K4me3 and/or H3K27me3 status between stem and progenitor cells as well as between progenitor and mature cells. Stem cell related pathways and proliferation or metabolism related pathways characterize genes differentially enriched for H3K4me3/H3K27me3 in stem and progenitor populations. Bivalent genes in stem cells are more plastic during differentiation and are more likely to lose H3K4me3 than to lose H3K27me3, consistent with increasingly closed chromatin state with differentiation. Our data indicates that histone modifications but not promoter DNA methylation are involved in switches from CSC s to non-CSC s in AML.

Original languageEnglish
Pages (from-to)92-104
Number of pages13
JournalEpigenetics
Volume8
Issue number1
DOIs
StatePublished - Jan 2013
Externally publishedYes

Keywords

  • AML
  • DNA methylation
  • Histone modification
  • Progenitor
  • Stem

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