TY - JOUR
T1 - The enhancement of osteogenesis through the use of dental pulp pluripotent stem cells in 3D
AU - Atari, M.
AU - Caballé-Serrano, J.
AU - Gil-Recio, C.
AU - Giner-Delgado, C.
AU - Martínez-Sarrà, E.
AU - García-Fernández, D. A.
AU - Barajas, M.
AU - Hernández-Alfaro, F.
AU - Ferrés-Padró, E.
AU - Giner-Tarrida, L.
N1 - Funding Information:
We thank M. Costa for help with the FACS analysis as well as J. Navarro and J. del Rey for their dedication during the cytogenetic analysis using a newly developed CGH technique [39] . This work was supported by the Universitat Internationl de Catalunya .
PY - 2012/4
Y1 - 2012/4
N2 - The potential for osteogenic differentiation of dental pulp mesenchymal stem cells (DPMSCs) in vitro and in vivo has been well documented in a variety of studies. Previously, we obtained a population of cells from human dental pulp called dental pulp pluripotent stem cells (DPPSCs) that could differentiate into mesodermal, ectodermal and endodermal progenies. We compared the osteogenic capacity of DPPSCs and DPMSCs that had been isolated from the same donors (N = 5) and cultivated in the same osteogenic medium in 3D (three dimensions) Cell Carrier glass scaffolds. We also compared the architecture of bone-like tissue obtained from DPPSCs and human maxillary bone tissue. Differentiation was evaluated by scanning electron microscopy, whereas the expression of bone markers such as ALP, Osteocalcin, COLL1 and Osteonectin was investigated by quantitative real time polymerase chain reaction (qRT-PCR). We also used calcium quantification, Alizarin red staining and alkaline phosphatase (ALP) activity to compare the two cell types. New bone tissue formed by DPPSCs was in perfect continuity with the trabecular host bone structure, and the restored bone network demonstrated high interconnectivity. Significant differences between DPPSCs and DPMSCs were observed for the expression of bone markers, calcium deposition and ALP activity during osteogenic differentiation; these criteria were higher for DPPSCs than DPMSCs. Both DPPSCs and differentiated tissue showed normal chromosomal dosage after being cultured in vitro and analysed using short-chromosome genomic hybridisation (short-CGH). This study demonstrates the stability and potential for the use of DPPSCs in bone tissue engineering applications.
AB - The potential for osteogenic differentiation of dental pulp mesenchymal stem cells (DPMSCs) in vitro and in vivo has been well documented in a variety of studies. Previously, we obtained a population of cells from human dental pulp called dental pulp pluripotent stem cells (DPPSCs) that could differentiate into mesodermal, ectodermal and endodermal progenies. We compared the osteogenic capacity of DPPSCs and DPMSCs that had been isolated from the same donors (N = 5) and cultivated in the same osteogenic medium in 3D (three dimensions) Cell Carrier glass scaffolds. We also compared the architecture of bone-like tissue obtained from DPPSCs and human maxillary bone tissue. Differentiation was evaluated by scanning electron microscopy, whereas the expression of bone markers such as ALP, Osteocalcin, COLL1 and Osteonectin was investigated by quantitative real time polymerase chain reaction (qRT-PCR). We also used calcium quantification, Alizarin red staining and alkaline phosphatase (ALP) activity to compare the two cell types. New bone tissue formed by DPPSCs was in perfect continuity with the trabecular host bone structure, and the restored bone network demonstrated high interconnectivity. Significant differences between DPPSCs and DPMSCs were observed for the expression of bone markers, calcium deposition and ALP activity during osteogenic differentiation; these criteria were higher for DPPSCs than DPMSCs. Both DPPSCs and differentiated tissue showed normal chromosomal dosage after being cultured in vitro and analysed using short-chromosome genomic hybridisation (short-CGH). This study demonstrates the stability and potential for the use of DPPSCs in bone tissue engineering applications.
KW - DPPSC
KW - Dental pulp stem cells
KW - Osteogenic differentiation
KW - Pluripotent stem cells
UR - http://www.scopus.com/inward/record.url?scp=84858072888&partnerID=8YFLogxK
U2 - 10.1016/j.bone.2012.01.005
DO - 10.1016/j.bone.2012.01.005
M3 - Article
C2 - 22270057
AN - SCOPUS:84858072888
SN - 8756-3282
VL - 50
SP - 930
EP - 941
JO - Bone
JF - Bone
IS - 4
ER -