The endocrine disrupting effects of sodium arsenite in the rat testis is not mediated through macrophage activation

Anderson Tadeu de Araújo-Ramos, Carla Giovana Basso, Marcella Tapias Passoni, Daniele Cristine Krebs Ribeiro, Katherinne Maria Spercoski, Jeane Maria de Oliveira, Renata Marino Romano, Camila Merino, Jéssica Maiara Marques Sandri, Mylla Freitas de Almeida, Fabrícia de Souza Predes, Anderson Joel Martino-Andrade

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2 Scopus citations


Arsenic (As) is an endocrine disrupting chemical that can disturb the male reproductive system. In a previous study, it was suggested that testicular macrophages could display a role in endocrine disruption induced by As exposure. This work aimed to evaluate the effects of chronic As exposure in the testis function of Wistar rats and examine the participation of macrophage activation and inflammatory response in these processes. We examined gene expression of steroidogenic machinery and immunological markers by RT-QPCR, plasma testosterone concentrations, sperm count and morphology, and histomorphometrical parameters after 60-days exposure to 1 or 5−−1 of sodium arsenite, combined or not with 50 μ−1 of LPS administered one day before euthanasia. We have demonstrated that As exposure reduced the weight of androgen-dependent organs and induced changes in spermatogenesis, in particular at the highest dose. LPS and As co-exposure promoted a decrease in testosterone synthesis, but did not increase the overexpression of markers of macrophage activation seen in LPS-only rats. Our results suggest that As does not alter the testicular macrophage function, but under immunological challenges LPS and As can display a complex interaction, which could lead to endocrine disruption.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalReproductive Toxicology
StatePublished - Jun 2021
Externally publishedYes


  • Arsenic
  • Endocrine-disrupting chemical
  • Endocrinology
  • Macrophage
  • Male reproductive system
  • Toxicology


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