TY - JOUR
T1 - The Effects of Topical and Oral L-Selenomethionine on Pigmentation and Skin Cancer Induced by Ultraviolet Irradiation
AU - Burke, Karen E.
AU - Combs, Gerald F.
AU - Gross, Earl G.
AU - Bhuyan, Kailash C.
AU - Abu-Libdeh, Hassan
N1 - Funding Information:
The authors thank Durga K. Bhuyan (Dept. of Ophthalmology, Mt. Sinai Medical Center, New York, NY) for helping with the eye examination of all the animals. They also thank Larry C. Clark (University of Arizona, Health Science Center, Tucson, AZ) for suggestions regarding the statistical analysis, Luis C. Calvo (presently with Estee Lauder, New York) for compounding the lotion with L-selenomethionine, and Yao-Fen Hu for technical assistance. The authors are most appreciative of Nutrition 21 (La Jolla, CA) for supplying the L-selenomethionine. This study was supported primarily by the Karen E. Burke Research Fund (New York) and in part by a grant from Germaine Monteil Cosmetiques (New York). Address reprint requests to Dr. K. E. Burke, The River Court, 429 East 52nd St., New York, NY 10022.
PY - 1992/1/1
Y1 - 1992/1/1
N2 - This study was conducted to determine whether oral and/or topical selenium (Se) supplementation can reduce the incidence of acute and/or chronic damage to the skin (i.e., sunburn and pigmentation and/or skin cancer, respectively) induced by ultraviolet (UV) irradiation in mice. Groups of 38 BALB:c female mice or 16 Skh:2 hairless pigmented mice were treated with 1) lotion vehicle, 2) 0.02% L-selenomethionine (SeMet) lotion, or 3) vehicle and 1.5 ppm SeMet in the drinking water. Within each group, 30 BALB:c mice or 12 Skh:2 mice were given UV irradiation (Westinghouse FS 40 bulbs) three times per week in doses of 0.575 and 0.24 J/cm2, respectively. The animals’ weights and food intakes and the Se concentrations of skin and liver were measured. Skin biopsies were taken from the backs and abdomens of all animals to evaluate the relative amounts of Se and the damage by UV irradiation, Skin pigmentation was scored, and the total number of clinically detectable skin tumors per animal was counted weekly. Results showed that the skin Se concentrations in areas of application of the lotion containing SeMet were greater than those of animals given comparable oral doses, while the Se concentrations of untreated skin and liver were similar to those of animals receiving oral Se. Mice treated with Se showed no signs of toxicity and had significantly less skin damage by UV irradiation, as indicated by reduced inflammation and pigmentation and by later onset and lesser incidence of skin cancer.
AB - This study was conducted to determine whether oral and/or topical selenium (Se) supplementation can reduce the incidence of acute and/or chronic damage to the skin (i.e., sunburn and pigmentation and/or skin cancer, respectively) induced by ultraviolet (UV) irradiation in mice. Groups of 38 BALB:c female mice or 16 Skh:2 hairless pigmented mice were treated with 1) lotion vehicle, 2) 0.02% L-selenomethionine (SeMet) lotion, or 3) vehicle and 1.5 ppm SeMet in the drinking water. Within each group, 30 BALB:c mice or 12 Skh:2 mice were given UV irradiation (Westinghouse FS 40 bulbs) three times per week in doses of 0.575 and 0.24 J/cm2, respectively. The animals’ weights and food intakes and the Se concentrations of skin and liver were measured. Skin biopsies were taken from the backs and abdomens of all animals to evaluate the relative amounts of Se and the damage by UV irradiation, Skin pigmentation was scored, and the total number of clinically detectable skin tumors per animal was counted weekly. Results showed that the skin Se concentrations in areas of application of the lotion containing SeMet were greater than those of animals given comparable oral doses, while the Se concentrations of untreated skin and liver were similar to those of animals receiving oral Se. Mice treated with Se showed no signs of toxicity and had significantly less skin damage by UV irradiation, as indicated by reduced inflammation and pigmentation and by later onset and lesser incidence of skin cancer.
UR - http://www.scopus.com/inward/record.url?scp=0026603547&partnerID=8YFLogxK
U2 - 10.1080/01635589209514180
DO - 10.1080/01635589209514180
M3 - Article
C2 - 1584707
AN - SCOPUS:0026603547
SN - 0163-5581
VL - 17
SP - 123
EP - 137
JO - Nutrition and Cancer
JF - Nutrition and Cancer
IS - 2
ER -