The effects of flash-annealing on glycerol kinase crystals

Ardeschir Vahedi-Faridi; Vivian Stojanoff; Joanne I. Yeh

doi:10.1107/S0907444905012746

The effects of flash-annealing on glycerol kinase crystals

Ardeschir Vahedi-Faridi
, Vivian Stojanoff
, Joanne I. Yeh

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Reflection profiles from glycerol kinase crystals were analyzed to determine the effect of flash-cooling on mosaicity (η) and peak intensity in order to reveal changes in mosaic domain structure and composition. The results showed that repeated flash-annealing causes a significant decrease in the averaged mosaicity along with an increase in the overall peak counts of reflections and an enhanced signal-to-noise ratio. Individual reflection-profile analysis revealed a mostly dual domain structure, showing the minimization of one domain as a result of flash-annealing.

Original language	English
Pages (from-to)	982-989
Number of pages	8
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	61
Issue number	7
DOIs	https://doi.org/10.1107/S0907444905012746
State	Published - Jul 2005
Externally published	Yes

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abstract = "Reflection profiles from glycerol kinase crystals were analyzed to determine the effect of flash-cooling on mosaicity (η) and peak intensity in order to reveal changes in mosaic domain structure and composition. The results showed that repeated flash-annealing causes a significant decrease in the averaged mosaicity along with an increase in the overall peak counts of reflections and an enhanced signal-to-noise ratio. Individual reflection-profile analysis revealed a mostly dual domain structure, showing the minimization of one domain as a result of flash-annealing.",

author = "Ardeschir Vahedi-Faridi and Vivian Stojanoff and Yeh, \{Joanne I.\}",

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AU - Stojanoff, Vivian

AU - Yeh, Joanne I.

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N2 - Reflection profiles from glycerol kinase crystals were analyzed to determine the effect of flash-cooling on mosaicity (η) and peak intensity in order to reveal changes in mosaic domain structure and composition. The results showed that repeated flash-annealing causes a significant decrease in the averaged mosaicity along with an increase in the overall peak counts of reflections and an enhanced signal-to-noise ratio. Individual reflection-profile analysis revealed a mostly dual domain structure, showing the minimization of one domain as a result of flash-annealing.

AB - Reflection profiles from glycerol kinase crystals were analyzed to determine the effect of flash-cooling on mosaicity (η) and peak intensity in order to reveal changes in mosaic domain structure and composition. The results showed that repeated flash-annealing causes a significant decrease in the averaged mosaicity along with an increase in the overall peak counts of reflections and an enhanced signal-to-noise ratio. Individual reflection-profile analysis revealed a mostly dual domain structure, showing the minimization of one domain as a result of flash-annealing.

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JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

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ER -

The effects of flash-annealing on glycerol kinase crystals

Abstract

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