TY - JOUR
T1 - The effect of scutellaria baicalensis on the signaling network in hepatocellular carcinoma cells
AU - Ye, Fei
AU - Che, Yufang
AU - McMillen, Elizabeth
AU - Gorski, Justin
AU - Brodman, Douglas
AU - Saw, Daisy
AU - Jiang, Bo
AU - Zhang, David Y.
N1 - Funding Information:
This study was partially supported by grants from Chinese American Medical Association (D. Y. Zhang and D. Saw) and the Mount Sinai School of Medicine (E. McMillen). The authors are grateful for Avi Maayan’s help on Genes2Networks analysis.
PY - 2009/7
Y1 - 2009/7
N2 - Scutellaria baicalensis is an anti-inflammatory and antineoplastic Chinese herbal therapy. We have previously shown that S. baicalensis can inhibit hepatocellular carcinoma (HCC) cell growth in vitro. In this study, we sought to determine the effect of S. baicalensis on the cell signaling network using our newly developed Pathway Array technology, which screens cell signaling pathways involved in cell cycle regulation. The HCC cell line (HepG2) was treated with S. baicalensis extract in vitro. The effect on the cell cycle was analyzed by flow cytometry, and the expression of various signaling proteins was assayed with Pathway Array. Our results indicate that S. baicalensis exerts a strong growth inhibition of the HepG2 cells via G2/M phase arrest. The Pathway Array analysis of 56 proteins revealed a total of 14 differentially expressed proteins or phosphorylations after treatment. Of these, 9 showed a dose-dependent decrease (p53, ETS1, Cdc25B, p63, EGFR, ERK1/2, XIAP, HIF-2, and Cdc25C) whereas one demonstrated a dose-dependent increase (Cyclin E) after treatment with 200 g/ml of S. baicalensis. Using computer simulation software, we identified additional hubs in the signaling network activated by S. baicalensis. These results indicate that S. baicalensis exerts a broad effect on cell signaling networks leading to a collective inhibition of cell proliferation.
AB - Scutellaria baicalensis is an anti-inflammatory and antineoplastic Chinese herbal therapy. We have previously shown that S. baicalensis can inhibit hepatocellular carcinoma (HCC) cell growth in vitro. In this study, we sought to determine the effect of S. baicalensis on the cell signaling network using our newly developed Pathway Array technology, which screens cell signaling pathways involved in cell cycle regulation. The HCC cell line (HepG2) was treated with S. baicalensis extract in vitro. The effect on the cell cycle was analyzed by flow cytometry, and the expression of various signaling proteins was assayed with Pathway Array. Our results indicate that S. baicalensis exerts a strong growth inhibition of the HepG2 cells via G2/M phase arrest. The Pathway Array analysis of 56 proteins revealed a total of 14 differentially expressed proteins or phosphorylations after treatment. Of these, 9 showed a dose-dependent decrease (p53, ETS1, Cdc25B, p63, EGFR, ERK1/2, XIAP, HIF-2, and Cdc25C) whereas one demonstrated a dose-dependent increase (Cyclin E) after treatment with 200 g/ml of S. baicalensis. Using computer simulation software, we identified additional hubs in the signaling network activated by S. baicalensis. These results indicate that S. baicalensis exerts a broad effect on cell signaling networks leading to a collective inhibition of cell proliferation.
UR - https://www.scopus.com/pages/publications/70449671065
U2 - 10.1080/01635580902803719
DO - 10.1080/01635580902803719
M3 - Article
C2 - 19838925
AN - SCOPUS:70449671065
SN - 0163-5581
VL - 61
SP - 530
EP - 537
JO - Nutrition and Cancer
JF - Nutrition and Cancer
IS - 4
ER -