The effect of pretreatment with pentobarbital on the extent of [14C] incorporation from [U-14C]glucose into various rat brain glycolytic intermediates: relevance to regulation at hexokinase and phosphofrucktokinase

L. P. Miller, S. Mayer, L. D. Braun, P. Geiger, W. H. Oldendorf

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

In the present investigation we monitored the incorporation of [14C] from [U-14C]glucose into various rat brain glycolytic intermediates of conscious and pentobarbital-anesthetized animals. Labeled glucose was delivered to brain by single bolus intracarotid injection and brain tissue was subsequently prepared at 15, 30 and 45 sec by freeze-blowing. Glycolytic intermediates were then separated by column chromatography. Our results showed a gradual decrease with time of14C-labeled glucose which gave a calculated rate for glucose metabolism of 0.86 μmol/min/g and 0.56 μmol/min/g in conscious and anesthetized animals, respectively. Compared to the results obtained using conscious animals the administration of pentobarbital not only resulted in a significant attenuation of the rate of glucose metabolism but also caused a similar reduction in the amount of14C incorporated into several glycolytic intermediates. These intermediates included: glucose 6-phosphate, fructose 6-phosphate, fructose, 1,6 diphosphate, dihydroxyacetone phosphate and post glycolytic compounds. In addition, pretreatment with pentobarbital resulted in a 75% increase in the endogenous concentration of glucose, 10% increase in glucose 6-phosphate, no change in fructose 6-phosphate and 42% decrease in lactate compared to levels in brains obtained from conscious animals. These results are discussed in relation to control of glycolysis through coupled regulation at hexokinase-phosphofructokinase.

Original languageEnglish
Pages (from-to)377-382
Number of pages6
JournalNeurochemical Research
Volume13
Issue number4
DOIs
StatePublished - Apr 1988
Externally publishedYes

Keywords

  • Rat brain
  • glycolysis regulation
  • pentobarbital

Fingerprint

Dive into the research topics of 'The effect of pretreatment with pentobarbital on the extent of [14C] incorporation from [U-14C]glucose into various rat brain glycolytic intermediates: relevance to regulation at hexokinase and phosphofrucktokinase'. Together they form a unique fingerprint.

Cite this