TY - JOUR
T1 - The drosophila selectin furrowed mediates intercellular planar cell polarity interactions via frizzled stabilization
AU - Chin, Mei Ling
AU - Mlodzik, Marek
N1 - Funding Information:
We are most grateful to Henry Sun for allowing M.-L.C. to complete all revisions needed in his laboratory. We thank the Bloomington Stock Center, Developmental Studies Hybridoma Bank, Hugo Bellen, and David Strutt for fly strains and antibodies. We thank J. Wu for sharing unpublished results on DE-cad-mediated S2 cell adhesion and J. Carvajal-Gonzalez, A.C. Roman, C. Iomini, and J. Wu for help with quantifications. We thank all Mlodzik laboratory members for discussions, R. Krauss, C. Pfleger, and R. Cagan for experimental suggestions, and G. Collu, W. Gault, L. Kelly, and U. Weber for comments on the manuscript. Confocal microscopy was performed at the Mount Sinai School of Medicine Microscopy Shared Resource Facility. This work was supported by the National Institutes of Health/National Institute of General Medical Sciences and National Eye Institute grants (to M.M.).
PY - 2013/9/16
Y1 - 2013/9/16
N2 - Establishment of planar cell polarity (PCP) in a tissue requires coordination of directional signals from cell to cell. It is thought that this is mediated by the core PCP factors, which include cell-adhesion molecules. Here, we demonstrate that furrowed, the Drosophila selectin, is required for PCP generation. Disruption of PCP in furrowed-deficient flies results from a primary defect in Fz levels and cell adhesion. Furrowed localizes at or near apical junctions, largely colocalizing with Frizzled and Flamingo (Fmi). It physically interacts with and stabilizes Frizzled, and it mediates intercellular Frizzled-Van Gogh (Vang)/Strabismus interactions, similarly to Fmi. Furrowed does so through a homophilic cell-adhesion role that is distinct from its known carbohydrate-binding function described during vertebrate blood-cell/endothelial cell interactions. Importantly, the carbohydrate function is dispensable for PCP establishment. Invivo studies suggest that Furrowed functions partially redundantly with Fmi, mediating intercellular Frizzled-Vang interactions between neighboring cells.
AB - Establishment of planar cell polarity (PCP) in a tissue requires coordination of directional signals from cell to cell. It is thought that this is mediated by the core PCP factors, which include cell-adhesion molecules. Here, we demonstrate that furrowed, the Drosophila selectin, is required for PCP generation. Disruption of PCP in furrowed-deficient flies results from a primary defect in Fz levels and cell adhesion. Furrowed localizes at or near apical junctions, largely colocalizing with Frizzled and Flamingo (Fmi). It physically interacts with and stabilizes Frizzled, and it mediates intercellular Frizzled-Van Gogh (Vang)/Strabismus interactions, similarly to Fmi. Furrowed does so through a homophilic cell-adhesion role that is distinct from its known carbohydrate-binding function described during vertebrate blood-cell/endothelial cell interactions. Importantly, the carbohydrate function is dispensable for PCP establishment. Invivo studies suggest that Furrowed functions partially redundantly with Fmi, mediating intercellular Frizzled-Vang interactions between neighboring cells.
UR - https://www.scopus.com/pages/publications/84884150646
U2 - 10.1016/j.devcel.2013.07.006
DO - 10.1016/j.devcel.2013.07.006
M3 - Article
C2 - 23973164
AN - SCOPUS:84884150646
SN - 1534-5807
VL - 26
SP - 455
EP - 468
JO - Developmental Cell
JF - Developmental Cell
IS - 5
ER -