The development of monoclonal human rabies virus-neutralizing antibodies as a substitute for pooled human immune globulin in the prophylactic treatment of rabies virus exposure

J. M. Champion, R. B. Kean, C. E. Rupprecht, A. L. Notkins, H. Koprowski, B. Dietzschold, D. C. Hooper

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

To provide a more defined and safer replacement for the human rabies immune globulin (HRIG) from pooled serum which is currently used for treatment of exposure to rabies virus we have developed a series of human rabies virus-specific monoclonal antibodies. Mouse-human heterohybrid myeloma cells producing rabies virus-specific human monoclonal antibodies were prepared using B cells obtained from volunteers recently-immunized with a commercial rabies virus vaccine (HDCV). Cell lines producing antibody which neutralized the Evelyn-Rokitnicki-Abelseth (ERA) rabies virus strain in vitro were cloned and the resulting monoclonal antibodies characterized for isotype, specificity against a variety of rabies virus isolates, and neutralization capacity. The ability of the monoclonal antibodies to neutralize a variety of rabies virus strains in vitro correlated with their binding specificity for these viruses in an enzyme-linked immunoadsorbant assay (ELISA). A number of these antibodies have proven suitable for the formulation of a prophylactic human monoclonal antibody-based reagent which would provide significant advantages to the HRIG in having defined, reproducible specificity, lessened possibility of contamination with viral pathogens, and consistent availability. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)81-90
Number of pages10
JournalJournal of Immunological Methods
Volume235
Issue number1-2
DOIs
StatePublished - 21 Feb 2000
Externally publishedYes

Keywords

  • Human monoclonal antibodies
  • Passive immunization
  • Rabies post-exposure prophylaxis
  • Rabies virus

Fingerprint

Dive into the research topics of 'The development of monoclonal human rabies virus-neutralizing antibodies as a substitute for pooled human immune globulin in the prophylactic treatment of rabies virus exposure'. Together they form a unique fingerprint.

Cite this