The DEK oncoprotein is a critical component of the EKLF/KLF1 enhancer in erythroid cells

Felix Lohmann, Mohan Dangeti, Shefali Soni, Xiaoyong Chen, Antanas Planutis, Margaret H. Baron, Kyunghee Choi, James J. Bieker

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Understanding how transcriptional regulators are themselves controlled is important in attaining a complete picture of the intracellular effects that follow signaling cascades during early development and cell-restricted differentiation. We have addressed this issue by focusing on the regulation of EKLF/KLF1, a zinc finger transcription factor that plays a necessary role in the global regulation of erythroid gene expression. Using biochemical affinity purification, we have identified the DEK oncoprotein as a critical factor that interacts with an essential upstream enhancer element of the EKLF promoter and exerts a positive effect on EKLF levels. This element also binds a core set of erythroid transcription factors, suggesting that DEK is part of a tissue-restricted enhanceosome that contains BMP4-dependent and -independent components. Together with local enrichment of properly coded histones and an open chromatin domain, optimal transcriptional activation of the EKLF locus can be established.

Original languageEnglish
Pages (from-to)3726-3738
Number of pages13
JournalMolecular and Cellular Biology
Volume35
Issue number21
DOIs
StatePublished - 2015

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