The crucial role of miR-126 on suppressing progression of esophageal cancer by targeting VEGF-A

Ranran Kong, Yuefeng Ma, Jie Feng, Shaomin Li, Wei Zhang, Jiantao Jiang, Jin Zhang, Zhe Qiao, Xiaoping Yang, Bin Zhou

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Background: miR-126 is a key regulator of oncogenic processes. It is functionally linked to cellular proliferation, survival and migration. Vascular endothelial growth factor A (VEGF-A), which is regarded as a tumorgenesis activator, could directly target miR-126 in several tumors. However, the mechanism in esophageal cancer remains unclear. Methods and results: In this study, the expression of miR-126 and VEGF-A were assessed in esophageal cancer tissues and esophageal cancer cell lines. We found that miR-126 has significantly lower expression in esophageal cancer tissues and esophageal cancer cell lines than in healthy tissues, while the expression of VEGF-A is high. Luciferase reporter assays were performed to investigate the relationship between VEGF-A and miR-126. We confirmed that VEGF-A is a target for miR-126. Furthermore, the proliferation of esophageal cancer cells with miR-126 overexpression and miR-126 knockdown was monitored using the MTT assay. The results showed that miR-126 could inhibit esophageal cancer cell proliferation in vitro. The effect of miR-126 was also detected in BALB/c nude mice with transplanted esophageal cancer cells. In vivo study showed that tumor growth was significantly suppressed by miR-126 overexpression. Conclusions: We believe that restoring miR-126 levels may be a promising therapeutic approach in cases of esophageal cancer.

Original languageEnglish
Article number3
JournalCellular and Molecular Biology Letters
Volume21
Issue number1
DOIs
StatePublished - 28 Jul 2016
Externally publishedYes

Keywords

  • Cell proliferation
  • Esophageal cancer
  • Lentivirus package
  • MTT assay
  • MiR-126
  • Tumorgenesis
  • VEGF-A
  • Xenograft model

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