The conserved RING-H2 finger of ROC1 is required for ubiquitin ligation

Angus Chen, Kenneth Wu, Serge Y. Fuchs, Peilin Tan, Carlos Gomez, Zhen Qiang Pan

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

ROC1 is a common component of a large family of ubiquitin E3 ligases that regulate cell cycle progression and signal transduction pathways. Here we present evidence suggesting that a conserved RING-H2 structure within ROC1 is critical for its ubiquitin ligation function. Mercury-containing sulfhydryl modification agents (ρ-hydroxymercuribenzoate and mercuric chloride) irreversibly inhibit the ROC1-CUL1 ubiquitin ligase activity without disrupting the complex. Consistent with this, these reagents also eliminate the ability of the Skp1-CUL1-HOS-ROC1 E3 ligase complex to support the ubiquitination of IκBα, site-directed mutagenesis analysis identifies RING-H2 finger residues Cys42, Cys45, Cys75, His77, His80, Cys83, Cys94, and Asp97 as being essential for the ROC1-dependent ubiquitin ligase activity. Furthermore, C42S/C45S and H80A mutations reduce the ability of ROC1 to interact with CUL1 in transfected cells and diminish the capacity of ROC1-CUL1 to form a stable complex with Cdc34 in vitro. However, C75S, H77A, C94S, and D97A substitutions have no detectable effect on ROC1 binding activities. Thus, the ROC1 RING-H2 finger may possess multiple biochemical properties that include stabilizing an interaction with CUL1 and recruiting Cdc34. A possible role of the RING finger in facilitating the Ub transfer reaction is discussed.

Original languageEnglish
Pages (from-to)15432-15439
Number of pages8
JournalJournal of Biological Chemistry
Volume275
Issue number20
DOIs
StatePublished - 19 May 2000

Fingerprint

Dive into the research topics of 'The conserved RING-H2 finger of ROC1 is required for ubiquitin ligation'. Together they form a unique fingerprint.

Cite this