The cholinergic inhibition of afterhyperpolarization in rat hippocampus is independent of cAMP-dependent protein kinase

Robert D. Blitzer; Tony Wong; Rabin Nouranifar; Emmanuel M. Landau

doi:10.1016/0006-8993(94)90096-5

The cholinergic inhibition of afterhyperpolarization in rat hippocampus is independent of cAMP-dependent protein kinase

Robert D. Blitzer, Tony Wong, Rabin Nouranifar, Emmanuel M. Landau

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The possible involvement of protein kinase A (PKA) in the muscarinic inhibition of the slow afterhyperpolarizing current (I_AHP) was investigated in rat hippocampal pyramidal cells. I_AHP) was recorded using the whole cell method in hippocampal slices, and Rp-cAMPs, a PKA antagonist, was applied intracellularly. The inhibition of I_AHP by carbachol was not affected by Rp-cAMPS. In contrast, Rp-cAMPS reduced the cAMP-dependent inhibition of I_AHP by norepinephrine. The results show that phosphorylation by PKA does not contribute to the muscarinic effect on I_AHP.

Original language	English
Pages (from-to)	312-314
Number of pages	3
Journal	Brain Research
Volume	646
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(94)90096-5
State	Published - 23 May 1994

Keywords

CAI
Carbachol
Norepinephrine
Rp-cAMPS
Whole cell recording
cAMP-dependent protein kinase

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10.1016/0006-8993(94)90096-5

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title = "The cholinergic inhibition of afterhyperpolarization in rat hippocampus is independent of cAMP-dependent protein kinase",

abstract = "The possible involvement of protein kinase A (PKA) in the muscarinic inhibition of the slow afterhyperpolarizing current (IAHP) was investigated in rat hippocampal pyramidal cells. IAHP) was recorded using the whole cell method in hippocampal slices, and Rp-cAMPs, a PKA antagonist, was applied intracellularly. The inhibition of IAHP by carbachol was not affected by Rp-cAMPS. In contrast, Rp-cAMPS reduced the cAMP-dependent inhibition of IAHP by norepinephrine. The results show that phosphorylation by PKA does not contribute to the muscarinic effect on IAHP.",

keywords = "CAI, Carbachol, Norepinephrine, Rp-cAMPS, Whole cell recording, cAMP-dependent protein kinase",

author = "Blitzer, {Robert D.} and Tony Wong and Rabin Nouranifar and Landau, {Emmanuel M.}",

note = "Funding Information: This research was supported by a grant from the National Alliance for Research on Schizophrenia and Affective Disorders (NARSAD) to R.D.B.",

year = "1994",

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doi = "10.1016/0006-8993(94)90096-5",

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pages = "312--314",

journal = "Brain Research",

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T1 - The cholinergic inhibition of afterhyperpolarization in rat hippocampus is independent of cAMP-dependent protein kinase

AU - Blitzer, Robert D.

AU - Wong, Tony

AU - Nouranifar, Rabin

AU - Landau, Emmanuel M.

N1 - Funding Information: This research was supported by a grant from the National Alliance for Research on Schizophrenia and Affective Disorders (NARSAD) to R.D.B.

PY - 1994/5/23

Y1 - 1994/5/23

N2 - The possible involvement of protein kinase A (PKA) in the muscarinic inhibition of the slow afterhyperpolarizing current (IAHP) was investigated in rat hippocampal pyramidal cells. IAHP) was recorded using the whole cell method in hippocampal slices, and Rp-cAMPs, a PKA antagonist, was applied intracellularly. The inhibition of IAHP by carbachol was not affected by Rp-cAMPS. In contrast, Rp-cAMPS reduced the cAMP-dependent inhibition of IAHP by norepinephrine. The results show that phosphorylation by PKA does not contribute to the muscarinic effect on IAHP.

AB - The possible involvement of protein kinase A (PKA) in the muscarinic inhibition of the slow afterhyperpolarizing current (IAHP) was investigated in rat hippocampal pyramidal cells. IAHP) was recorded using the whole cell method in hippocampal slices, and Rp-cAMPs, a PKA antagonist, was applied intracellularly. The inhibition of IAHP by carbachol was not affected by Rp-cAMPS. In contrast, Rp-cAMPS reduced the cAMP-dependent inhibition of IAHP by norepinephrine. The results show that phosphorylation by PKA does not contribute to the muscarinic effect on IAHP.

KW - CAI

KW - Carbachol

KW - Norepinephrine

KW - Rp-cAMPS

KW - Whole cell recording

KW - cAMP-dependent protein kinase

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SN - 0006-8993

VL - 646

SP - 312

EP - 314

JO - Brain Research

JF - Brain Research

IS - 2

ER -

The cholinergic inhibition of afterhyperpolarization in rat hippocampus is independent of cAMP-dependent protein kinase

Abstract

Keywords

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