TY - JOUR
T1 - The Bacillus subtilis Quorum-Sensing Molecule CSF Contributes to Intestinal Homeostasis via OCTN2, a Host Cell Membrane Transporter
AU - Fujiya, Mikihiro
AU - Musch, Mark W.
AU - Nakagawa, Yasushi
AU - Hu, Shien
AU - Alverdy, John
AU - Kohgo, Yutaka
AU - Schneewind, Olaf
AU - Jabri, Bana
AU - Chang, Eugene B.
N1 - Funding Information:
We would like to thank Dr. Stephen Hanauer for his support and for providing clinical specimens. The study was supported by the Digestive Disease Research Core Center of the University of Chicago (DK42086) and by grants from the following sources: Crohn's and Colitis Foundation of America (E.B.C.), NIH DK47722 (E.B.C.), NIH DK38510 (E.B.C.), and Grant-in-Aid for Scientific Research 18590667 (M.F.) Special thanks to the Gastrointestinal Research Foundation, Martin Boyer, and Marian Edelstein for support of the research laboratories and programs, and to Alexander Chervonsky for reviewing the manuscript.
PY - 2007/6/14
Y1 - 2007/6/14
N2 - Bacteria use quorum-sensing molecules (QSMs) to communicate within as well as across species. However, the effects of QSMs on eukaryotic host cells have received limited attention. We report that the quorum-sensing pentapeptide, competence and sporulation factor (CSF), of the Gram-positive bacterium Bacillus subtilis activates key survival pathways, including p38 MAP kinase and protein kinase B (Akt), in intestinal epithelial cells. CSF also induces cytoprotective heat shock proteins (Hsps), which prevent oxidant-induced intestinal epithelial cell injury and loss of barrier function. These effects of CSF depend on its uptake by an apical membrane organic cation transporter-2 (OCTN2). Thus, OCTN2-mediated CSF transport serves as an example of a host-bacterial interaction that allows the host to monitor and respond to changes in the behavior or composition of colonic flora.
AB - Bacteria use quorum-sensing molecules (QSMs) to communicate within as well as across species. However, the effects of QSMs on eukaryotic host cells have received limited attention. We report that the quorum-sensing pentapeptide, competence and sporulation factor (CSF), of the Gram-positive bacterium Bacillus subtilis activates key survival pathways, including p38 MAP kinase and protein kinase B (Akt), in intestinal epithelial cells. CSF also induces cytoprotective heat shock proteins (Hsps), which prevent oxidant-induced intestinal epithelial cell injury and loss of barrier function. These effects of CSF depend on its uptake by an apical membrane organic cation transporter-2 (OCTN2). Thus, OCTN2-mediated CSF transport serves as an example of a host-bacterial interaction that allows the host to monitor and respond to changes in the behavior or composition of colonic flora.
KW - MICROBIO
UR - http://www.scopus.com/inward/record.url?scp=34249893108&partnerID=8YFLogxK
U2 - 10.1016/j.chom.2007.05.004
DO - 10.1016/j.chom.2007.05.004
M3 - Article
C2 - 18005709
AN - SCOPUS:34249893108
SN - 1931-3128
VL - 1
SP - 299
EP - 308
JO - Cell Host and Microbe
JF - Cell Host and Microbe
IS - 4
ER -