The Analysis of Natural Killer Cell Activity by Flow Cytometry

Tamas Racz; Peter Sacks; Nguyan T. Van; Dorothy L. Taylor; Greg Young; Samuel Bugis; Howard E. Savage; Stimson P. Schantz

doi:10.1001/archotol.1990.01870040062015

The Analysis of Natural Killer Cell Activity by Flow Cytometry

Tamas Racz
, Peter Sacks
, Nguyan T. Van
, Dorothy L. Taylor
, Greg Young
, Samuel Bugis
, Howard E. Savage
, Stimson P. Schantz

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A flow cytometric assay was used to detect the lytic and binding capacities of both fresh peripheral blood lymphocytes and purified Leu-19⁺ natural killer cells against head and neck cancer cell lines. Results demonstrated that natural killer cell-mediated cytotoxicity and effector-target conjugate formation evaluated by flow cytometry was significantly correlated with the standard chromium 51 release assay and the single-cell microscopic assay, respectively. The sorted Leu-19⁺ natural killer cells demonstrated higher lytic capacity with a corresponding higher binding rate compared with the unsorted peripheral blood lymphocytes and sorted Leu-19⁻ cells. Flow cytometric analysis of natural killer cell activity (a rapid, simple, and quantifiable procedure) is an alternative to the standard chromium 51 release assay. (Arch Otolaryngol Head Neck Surg. 1990;116:440-446).

Original language	English
Pages (from-to)	440-446
Number of pages	7
Journal	JAMA Otolaryngology - Head and Neck Surgery
Volume	116
Issue number	4
DOIs	https://doi.org/10.1001/archotol.1990.01870040062015
State	Published - Apr 1990
Externally published	Yes

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title = "The Analysis of Natural Killer Cell Activity by Flow Cytometry",

abstract = "A flow cytometric assay was used to detect the lytic and binding capacities of both fresh peripheral blood lymphocytes and purified Leu-19+ natural killer cells against head and neck cancer cell lines. Results demonstrated that natural killer cell-mediated cytotoxicity and effector-target conjugate formation evaluated by flow cytometry was significantly correlated with the standard chromium 51 release assay and the single-cell microscopic assay, respectively. The sorted Leu-19+ natural killer cells demonstrated higher lytic capacity with a corresponding higher binding rate compared with the unsorted peripheral blood lymphocytes and sorted Leu-19− cells. Flow cytometric analysis of natural killer cell activity (a rapid, simple, and quantifiable procedure) is an alternative to the standard chromium 51 release assay. (Arch Otolaryngol Head Neck Surg. 1990;116:440-446).",

author = "Tamas Racz and Peter Sacks and Van, \{Nguyan T.\} and Taylor, \{Dorothy L.\} and Greg Young and Samuel Bugis and Savage, \{Howard E.\} and Schantz, \{Stimson P.\}",

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language = "English",

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TY - JOUR

T1 - The Analysis of Natural Killer Cell Activity by Flow Cytometry

AU - Racz, Tamas

AU - Sacks, Peter

AU - Van, Nguyan T.

AU - Taylor, Dorothy L.

AU - Young, Greg

AU - Bugis, Samuel

AU - Savage, Howard E.

AU - Schantz, Stimson P.

PY - 1990/4

Y1 - 1990/4

N2 - A flow cytometric assay was used to detect the lytic and binding capacities of both fresh peripheral blood lymphocytes and purified Leu-19+ natural killer cells against head and neck cancer cell lines. Results demonstrated that natural killer cell-mediated cytotoxicity and effector-target conjugate formation evaluated by flow cytometry was significantly correlated with the standard chromium 51 release assay and the single-cell microscopic assay, respectively. The sorted Leu-19+ natural killer cells demonstrated higher lytic capacity with a corresponding higher binding rate compared with the unsorted peripheral blood lymphocytes and sorted Leu-19− cells. Flow cytometric analysis of natural killer cell activity (a rapid, simple, and quantifiable procedure) is an alternative to the standard chromium 51 release assay. (Arch Otolaryngol Head Neck Surg. 1990;116:440-446).

AB - A flow cytometric assay was used to detect the lytic and binding capacities of both fresh peripheral blood lymphocytes and purified Leu-19+ natural killer cells against head and neck cancer cell lines. Results demonstrated that natural killer cell-mediated cytotoxicity and effector-target conjugate formation evaluated by flow cytometry was significantly correlated with the standard chromium 51 release assay and the single-cell microscopic assay, respectively. The sorted Leu-19+ natural killer cells demonstrated higher lytic capacity with a corresponding higher binding rate compared with the unsorted peripheral blood lymphocytes and sorted Leu-19− cells. Flow cytometric analysis of natural killer cell activity (a rapid, simple, and quantifiable procedure) is an alternative to the standard chromium 51 release assay. (Arch Otolaryngol Head Neck Surg. 1990;116:440-446).

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DO - 10.1001/archotol.1990.01870040062015

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JO - JAMA Otolaryngology - Head and Neck Surgery

JF - JAMA Otolaryngology - Head and Neck Surgery

IS - 4

ER -

The Analysis of Natural Killer Cell Activity by Flow Cytometry

Abstract

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