The Analysis of Natural Killer Cell Activity by Flow Cytometry

Tamas Racz, Peter Sacks, Nguyan T. Van, Dorothy L. Taylor, Greg Young, Samuel Bugis, Howard E. Savage, Stimson P. Schantz

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A flow cytometric assay was used to detect the lytic and binding capacities of both fresh peripheral blood lymphocytes and purified Leu-19+ natural killer cells against head and neck cancer cell lines. Results demonstrated that natural killer cell-mediated cytotoxicity and effector-target conjugate formation evaluated by flow cytometry was significantly correlated with the standard chromium 51 release assay and the single-cell microscopic assay, respectively. The sorted Leu-19+ natural killer cells demonstrated higher lytic capacity with a corresponding higher binding rate compared with the unsorted peripheral blood lymphocytes and sorted Leu-19 cells. Flow cytometric analysis of natural killer cell activity (a rapid, simple, and quantifiable procedure) is an alternative to the standard chromium 51 release assay. (Arch Otolaryngol Head Neck Surg. 1990;116:440-446).

Original languageEnglish
Pages (from-to)440-446
Number of pages7
JournalJAMA Otolaryngology - Head and Neck Surgery
Volume116
Issue number4
DOIs
StatePublished - Apr 1990
Externally publishedYes

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