Abstract
We have studied a human neuroblastoma cell line containing a homogeneously staining region (HSR) on the short (p) arm of chromosome #13. The HSRs are believed to represent sites of gene amplification, containing multiple copies of one or a small number of genes. The 18S and 28S ribosomal RNA genes are the only structural genes so far assigned to the p arms of the human acrocentric chromosomes, which include number #13. Using two recombinant DNA probes specific for sequences in the 18S/28S ribosomal RNA (rRNA) gene complex for in situ hybridization, we have been able to establish that the 13pHSR in this line does not contain rRNA genes.
Original language | English |
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Pages (from-to) | 113-119 |
Number of pages | 7 |
Journal | Cancer Genetics and Cytogenetics |
Volume | 11 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1984 |