TY - JOUR
T1 - TGF-β-induced macrophage colony-stimulating factor gene expression in various mesenchymal cell lines
AU - Takaishi, T.
AU - Matsui, T.
AU - Tsukamoto, T.
AU - Ito, M.
AU - Taniguchi, T.
AU - Fukase, M.
AU - Chihara, K.
PY - 1994
Y1 - 1994
N2 - We report here that transforming growth factor-β (TGF-β) can increase the expression level of macrophage colony-stimulating factor (M-CSF) mRNA in a variety of mesenchymal cell lines derived from osteoblasts, bone marrow stromal cells, fibroblasts, and myoblasts. The M-CSF activity in the conditioned medium of mouse osteoblast-like MC3T3-E1 cells was increased by TGF-β as well as interleukin-1 (IL-1) treatment. The increase of M-CSF mRNA expression was observed as early as 2 h after TGF-β or IL-1 addition and was superinduced by cycloheximide treatment. Nuclear run-off assays revealed that the increase in M-CSF mRNA by TGF-β as well as IL-1 occurred, at least in part, at the transcriptional level. Platelet-derived growth factor (PDGF) also enhanced the M-CSF production in MC3T3-E1 cells. Furthermore, TGF-β and IL-1 distinctly induced both PDGF-A and PDGF-B chain mRNA in MC3T3-E1 with different time courses. Our present studies suggest that PDGF autocrine loop- dependent and loop-independent pathways could modulate the M-CSF production stimulated by TGF-β or IL-1 and account for the complexity of the cytokine network involving M-CSF in vivo under various physiological and pathological conditions.
AB - We report here that transforming growth factor-β (TGF-β) can increase the expression level of macrophage colony-stimulating factor (M-CSF) mRNA in a variety of mesenchymal cell lines derived from osteoblasts, bone marrow stromal cells, fibroblasts, and myoblasts. The M-CSF activity in the conditioned medium of mouse osteoblast-like MC3T3-E1 cells was increased by TGF-β as well as interleukin-1 (IL-1) treatment. The increase of M-CSF mRNA expression was observed as early as 2 h after TGF-β or IL-1 addition and was superinduced by cycloheximide treatment. Nuclear run-off assays revealed that the increase in M-CSF mRNA by TGF-β as well as IL-1 occurred, at least in part, at the transcriptional level. Platelet-derived growth factor (PDGF) also enhanced the M-CSF production in MC3T3-E1 cells. Furthermore, TGF-β and IL-1 distinctly induced both PDGF-A and PDGF-B chain mRNA in MC3T3-E1 with different time courses. Our present studies suggest that PDGF autocrine loop- dependent and loop-independent pathways could modulate the M-CSF production stimulated by TGF-β or IL-1 and account for the complexity of the cytokine network involving M-CSF in vivo under various physiological and pathological conditions.
KW - interleukin-1
KW - osteoblast
KW - platelet-derived growth factor
KW - transforming growth factor-β
UR - http://www.scopus.com/inward/record.url?scp=0028071532&partnerID=8YFLogxK
U2 - 10.1152/ajpcell.1994.267.1.c25
DO - 10.1152/ajpcell.1994.267.1.c25
M3 - Article
C2 - 8048485
AN - SCOPUS:0028071532
SN - 0363-6143
VL - 267
SP - C25-C31
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
IS - 1 36-1
ER -