@article{d9beb2cf5129443eada98b8e6275833c,
title = "Tex10 Coordinates Epigenetic Control of Super-Enhancer Activity in Pluripotency and Reprogramming",
abstract = "Super-enhancers (SEs) are large clusters of transcriptional enhancers that are co-occupied by multiple lineage-specific transcription factors driving expression of genes that define cell identity. In embryonic stem cells (ESCs), SEs are highly enriched for the core pluripotency factors Oct4, Sox2, and Nanog. In this study, we sought to dissect the molecular control mechanism of SE activity in pluripotency and reprogramming. Starting from a protein interaction network surrounding Sox2, we identified Tex10 as a key pluripotency factor that plays a functionally significant role in ESC self-renewal, early embryo development, and reprogramming. Tex10 is enriched at SEs in a Sox2-dependent manner and coordinates histone acetylation and DNA demethylation at SEs. Tex10 activity is also important for pluripotency and reprogramming in human cells. Our study therefore highlights Tex10 as a core component of the pluripotency network and sheds light on its role in epigenetic control of SE activity for cell fate determination.",
author = "Junjun Ding and Xin Huang and Ningyi Shao and Hongwei Zhou and Lee, {Dung Fang} and Francesco Faiola and Miguel Fidalgo and Diana Guallar and Arven Saunders and Shliaha, {Pavel V.} and Hailong Wang and Avinash Waghray and Dmitri Papatsenko and Carlos S{\'a}nchez-Priego and Dan Li and Ye Yuan and Lemischka, {Ihor R.} and Li Shen and Kevin Kelley and Haiteng Deng and Xiaohua Shen and Jianlong Wang",
note = "Funding Information: We thank Dr. Jose Silva (United Kingdom) for the pre-iPSCs; Dr. Hitoshi Niwa (Japan) for the 2TS22C ESCs; Dr. Thamar van Dijk (The Nethelands) for Wdr18, Tex10, and Las1L plasmids; and Dr. Huck-Hui Ng (Singapore) for the Nanog -enhancer luciferase construct. Production of the knockout mouse model and injections of siRNAs in early embryos were performed in the Mouse Genetics Shared Resource Facility at the Icahn School of Medicine at Mount Sinai. This research was funded by grants from the NIH to J.W. (1R01-GM095942) and the Empire State Stem Cell Fund through New York State Department of Health (NYSTEM) to J.W. (C028103 and C028121). J.W. is a recipient of an Irma T. Hirschl and Weill-Caulier Trusts Career Scientist Award. A.S. is an awardee of the Traineeship of NIDCR-Interdisciplinary Training in Systems and Developmental Biology and Birth Defects (T32HD075735). Funding Information: We thank Dr. Jose Silva (United Kingdom) for the pre-iPSCs; Dr. Hitoshi Niwa (Japan) for the 2TS22C ESCs; Dr. Thamar van Dijk (The Nethelands) for Wdr18, Tex10, and Las1L plasmids; and Dr. Huck-Hui Ng (Singapore) for the Nanogenhancer luciferase construct. Production of the knockout mouse model and injections of siRNAs in early embryos were performed in the Mouse Genetics Shared Resource Facility at the Icahn School of Medicine at Mount Sinai. This research was funded by grants from the NIH to J.W. (1R01-GM095942) and the Empire State Stem Cell Fund through New York State Department of Health (NYSTEM) to J.W. (C028103 and C028121). J.W. is a recipient of an Irma T. Hirschl and Weill-Caulier Trusts Career Scientist Award. A.S. is an awardee of the Traineeship of NIDCR-Interdisciplinary Training in Systems and Developmental Biology and Birth Defects (T32HD075735). Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",
year = "2015",
month = jun,
day = "4",
doi = "10.1016/j.stem.2015.04.001",
language = "English",
volume = "16",
pages = "653--668",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "6",
}