Tetracyclines modulate cytosolic Ca2+ responses in the osteoclast associated with "Ca2+ receptor" activation

Christopher M.R. Bax; Vijai S. Shankar; A. S.M. Towhidul Alam; Bridget E. Bax; Baljit S. Moonga; Christopher L.H. Huang; Mone Zaidi; Barry R. Rifkin

doi:10.1007/BF01149961

Tetracyclines modulate cytosolic Ca²⁺ responses in the osteoclast associated with "Ca²⁺ receptor" activation

Christopher M.R. Bax, Vijai S. Shankar, A. S.M. Towhidul Alam, Bridget E. Bax, Baljit S. Moonga, Christopher L.H. Huang, Mone Zaidi, Barry R. Rifkin

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We report the effects of tetracycline analogues on cytosolic Ca²⁺ transients resulting from application of ionic nickel (Ni²⁺), a potent surrogate agonist of the osteoclast Ca²⁺ "receptor". Preincubation with minocycline (1 mg/l) or a chemically modified tetracycline, 4-dedimethyl-aminotetracycline (CMT-1) (1 or 10 mg/l), resulted in a significant attenuation of the magnitude of the cytosolic [Ca²⁺] response to an application of 5 mM-[Ni²⁺]. Preincubation with doxycycline (1 or 10 mg/l) failed to produce similar results. In addition, application of minocycline alone (0.1-100 mg/l) resulted in a 3.5-fold elevation of cytosolic [Ca²⁺]. The results suggest a novel action of tetracyclines on the osteoclast Ca²⁺ "receptor".

Original language	English
Pages (from-to)	169-174
Number of pages	6
Journal	Bioscience Reports
Volume	13
Issue number	3
DOIs	https://doi.org/10.1007/BF01149961
State	Published - Jun 1993
Externally published	Yes

Keywords

cytosolic Ca
osteoclasts
tetracycline

Access to Document

10.1007/BF01149961

Cite this

@article{1a1b9efe7ef947449e3b46acac02a99a,

title = "Tetracyclines modulate cytosolic Ca2+ responses in the osteoclast associated with {"}Ca2+ receptor{"} activation",

abstract = "We report the effects of tetracycline analogues on cytosolic Ca2+ transients resulting from application of ionic nickel (Ni2+), a potent surrogate agonist of the osteoclast Ca2+ {"}receptor{"}. Preincubation with minocycline (1 mg/l) or a chemically modified tetracycline, 4-dedimethyl-aminotetracycline (CMT-1) (1 or 10 mg/l), resulted in a significant attenuation of the magnitude of the cytosolic [Ca2+] response to an application of 5 mM-[Ni2+]. Preincubation with doxycycline (1 or 10 mg/l) failed to produce similar results. In addition, application of minocycline alone (0.1-100 mg/l) resulted in a 3.5-fold elevation of cytosolic [Ca2+]. The results suggest a novel action of tetracyclines on the osteoclast Ca2+ {"}receptor{"}.",

keywords = "cytosolic Ca, osteoclasts, tetracycline",

author = "Bax, {Christopher M.R.} and Shankar, {Vijai S.} and {Towhidul Alam}, {A. S.M.} and Bax, {Bridget E.} and Moonga, {Baljit S.} and Huang, {Christopher L.H.} and Mone Zaidi and Rifkin, {Barry R.}",

year = "1993",

month = jun,

doi = "10.1007/BF01149961",

language = "English",

volume = "13",

pages = "169--174",

journal = "Bioscience Reports",

issn = "0144-8463",

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TY - JOUR

T1 - Tetracyclines modulate cytosolic Ca2+ responses in the osteoclast associated with "Ca2+ receptor" activation

AU - Bax, Christopher M.R.

AU - Shankar, Vijai S.

AU - Towhidul Alam, A. S.M.

AU - Bax, Bridget E.

AU - Moonga, Baljit S.

AU - Huang, Christopher L.H.

AU - Zaidi, Mone

AU - Rifkin, Barry R.

PY - 1993/6

Y1 - 1993/6

N2 - We report the effects of tetracycline analogues on cytosolic Ca2+ transients resulting from application of ionic nickel (Ni2+), a potent surrogate agonist of the osteoclast Ca2+ "receptor". Preincubation with minocycline (1 mg/l) or a chemically modified tetracycline, 4-dedimethyl-aminotetracycline (CMT-1) (1 or 10 mg/l), resulted in a significant attenuation of the magnitude of the cytosolic [Ca2+] response to an application of 5 mM-[Ni2+]. Preincubation with doxycycline (1 or 10 mg/l) failed to produce similar results. In addition, application of minocycline alone (0.1-100 mg/l) resulted in a 3.5-fold elevation of cytosolic [Ca2+]. The results suggest a novel action of tetracyclines on the osteoclast Ca2+ "receptor".

AB - We report the effects of tetracycline analogues on cytosolic Ca2+ transients resulting from application of ionic nickel (Ni2+), a potent surrogate agonist of the osteoclast Ca2+ "receptor". Preincubation with minocycline (1 mg/l) or a chemically modified tetracycline, 4-dedimethyl-aminotetracycline (CMT-1) (1 or 10 mg/l), resulted in a significant attenuation of the magnitude of the cytosolic [Ca2+] response to an application of 5 mM-[Ni2+]. Preincubation with doxycycline (1 or 10 mg/l) failed to produce similar results. In addition, application of minocycline alone (0.1-100 mg/l) resulted in a 3.5-fold elevation of cytosolic [Ca2+]. The results suggest a novel action of tetracyclines on the osteoclast Ca2+ "receptor".

KW - cytosolic Ca

KW - osteoclasts

KW - tetracycline

UR - http://www.scopus.com/inward/record.url?scp=0027372413&partnerID=8YFLogxK

U2 - 10.1007/BF01149961

DO - 10.1007/BF01149961

M3 - Article

C2 - 8268424

AN - SCOPUS:0027372413

SN - 0144-8463

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SP - 169

EP - 174

JO - Bioscience Reports

JF - Bioscience Reports

IS - 3