tetA(L) mutants of a tetracycline-sensitive strain of bacillus subtilis with the polynucleotide phosphorylase gene deleted

David H. Bechhofer; Stan J. Stasinopoulos

doi:10.1128/jb.180.13.3470-3473.1998

tetA(L) mutants of a tetracycline-sensitive strain of bacillus subtilis with the polynucleotide phosphorylase gene deleted

David H. Bechhofer, Stan J. Stasinopoulos

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A Bacillus subtilis strain with the polynucleotide phosphorylase gene deleted was sensitive for growth in the presence of tetracycline. This strain was used to select for tetracycline-resistant mutants. A point mutation in the tetA(L) promoter and a spontaneously occurring tetA(L) gene copy number mutant were characterized.

Original language	English
Pages (from-to)	3470-3473
Number of pages	4
Journal	Journal of Bacteriology
Volume	180
Issue number	13
DOIs	https://doi.org/10.1128/jb.180.13.3470-3473.1998
State	Published - Jul 1998
Externally published	Yes

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10.1128/jb.180.13.3470-3473.1998

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title = "tetA(L) mutants of a tetracycline-sensitive strain of bacillus subtilis with the polynucleotide phosphorylase gene deleted",

abstract = "A Bacillus subtilis strain with the polynucleotide phosphorylase gene deleted was sensitive for growth in the presence of tetracycline. This strain was used to select for tetracycline-resistant mutants. A point mutation in the tetA(L) promoter and a spontaneously occurring tetA(L) gene copy number mutant were characterized.",

author = "Bechhofer, {David H.} and Stasinopoulos, {Stan J.}",

year = "1998",

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AU - Bechhofer, David H.

AU - Stasinopoulos, Stan J.

PY - 1998/7

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N2 - A Bacillus subtilis strain with the polynucleotide phosphorylase gene deleted was sensitive for growth in the presence of tetracycline. This strain was used to select for tetracycline-resistant mutants. A point mutation in the tetA(L) promoter and a spontaneously occurring tetA(L) gene copy number mutant were characterized.

AB - A Bacillus subtilis strain with the polynucleotide phosphorylase gene deleted was sensitive for growth in the presence of tetracycline. This strain was used to select for tetracycline-resistant mutants. A point mutation in the tetA(L) promoter and a spontaneously occurring tetA(L) gene copy number mutant were characterized.

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U2 - 10.1128/jb.180.13.3470-3473.1998

DO - 10.1128/jb.180.13.3470-3473.1998

M3 - Article

C2 - 9642204

AN - SCOPUS:0031749890

SN - 0021-9193

VL - 180

SP - 3470

EP - 3473

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 13

ER -

tetA(L) mutants of a tetracycline-sensitive strain of bacillus subtilis with the polynucleotide phosphorylase gene deleted

Abstract

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