Terpyridyl oxovanadium(IV) complexes for DNA crosslinking and mito-targeted photocytotoxicity

Arun Kumar; Ila Pant; Akanksha Dixit; Samya Banerjee; Bhabatosh Banik; Rupak Saha; Paturu Kondaiah; Akhil R. Chakravarty

doi:10.1016/j.jinorgbio.2017.05.015

Terpyridyl oxovanadium(IV) complexes for DNA crosslinking and mito-targeted photocytotoxicity

Arun Kumar, Ila Pant, Akanksha Dixit, Samya Banerjee, Bhabatosh Banik, Rupak Saha, Paturu Kondaiah, Akhil R. Chakravarty

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Oxovanadium(IV) complexes [VO(L¹/L²)Cl₂]^{n +} (1,2) of (anthracenyl)terpyridine (An-tpy as L¹ in 1, n = 0) and triphenylphosphonium-appended (anthracenyl)terpyridine (An-tpy-TPP⁺ as L² in 2, n = 1) were synthesized, characterized and their DNA crosslinking ability, photocytotoxicity in visible light and cellular localization in cancer cells studied. The bromide derivative of 2, viz. [VO(An-tpy-TPP)Br₂]Br (3) is structurally characterized. The structure showed trans disposition of two halides in the coordination sphere and the TPP⁺ unit is a pendant to the terpyridyl ligand. The DNA melting and comet assay studies on the complexes suggest the formation of DNA crosslinks. Complexes 1 and 2 displayed ~ 10 fold increase in cytotoxicity on exposure to visible light (400–700 nm) when compared to those in dark in HeLa and MCF-7 cells. FACScan (Fluorescence Associated Cell Sorter Scan) analysis showed cellular apoptosis when treated with the complex in visible light in comparison to their dark controls. Fluorescence microscopic studies using complex 2 revealed its mitochondrial localization within the cancer cells.

Original language	English
Pages (from-to)	45-54
Number of pages	10
Journal	Journal of Inorganic Biochemistry
Volume	174
DOIs	https://doi.org/10.1016/j.jinorgbio.2017.05.015
State	Published - Sep 2017
Externally published	Yes

Keywords

Crystal structure
DNA crosslinking
Mitochondria targeting
Photocytotoxicity
Terpyridine derivative
Vanadium

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10.1016/j.jinorgbio.2017.05.015

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@article{356853030a9b49a3817918ee70f271c9,

title = "Terpyridyl oxovanadium(IV) complexes for DNA crosslinking and mito-targeted photocytotoxicity",

abstract = "Oxovanadium(IV) complexes [VO(L1/L2)Cl2]n + (1,2) of (anthracenyl)terpyridine (An-tpy as L1 in 1, n = 0) and triphenylphosphonium-appended (anthracenyl)terpyridine (An-tpy-TPP+ as L2 in 2, n = 1) were synthesized, characterized and their DNA crosslinking ability, photocytotoxicity in visible light and cellular localization in cancer cells studied. The bromide derivative of 2, viz. [VO(An-tpy-TPP)Br2]Br (3) is structurally characterized. The structure showed trans disposition of two halides in the coordination sphere and the TPP+ unit is a pendant to the terpyridyl ligand. The DNA melting and comet assay studies on the complexes suggest the formation of DNA crosslinks. Complexes 1 and 2 displayed ~ 10 fold increase in cytotoxicity on exposure to visible light (400–700 nm) when compared to those in dark in HeLa and MCF-7 cells. FACScan (Fluorescence Associated Cell Sorter Scan) analysis showed cellular apoptosis when treated with the complex in visible light in comparison to their dark controls. Fluorescence microscopic studies using complex 2 revealed its mitochondrial localization within the cancer cells.",

keywords = "Crystal structure, DNA crosslinking, Mitochondria targeting, Photocytotoxicity, Terpyridine derivative, Vanadium",

author = "Arun Kumar and Ila Pant and Akanksha Dixit and Samya Banerjee and Bhabatosh Banik and Rupak Saha and Paturu Kondaiah and Chakravarty, {Akhil R.}",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier Inc.",

year = "2017",

month = sep,

doi = "10.1016/j.jinorgbio.2017.05.015",

language = "English",

volume = "174",

pages = "45--54",

journal = "Journal of Inorganic Biochemistry",

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T1 - Terpyridyl oxovanadium(IV) complexes for DNA crosslinking and mito-targeted photocytotoxicity

AU - Kumar, Arun

AU - Pant, Ila

AU - Dixit, Akanksha

AU - Banerjee, Samya

AU - Banik, Bhabatosh

AU - Saha, Rupak

AU - Kondaiah, Paturu

AU - Chakravarty, Akhil R.

PY - 2017/9

Y1 - 2017/9

N2 - Oxovanadium(IV) complexes [VO(L1/L2)Cl2]n + (1,2) of (anthracenyl)terpyridine (An-tpy as L1 in 1, n = 0) and triphenylphosphonium-appended (anthracenyl)terpyridine (An-tpy-TPP+ as L2 in 2, n = 1) were synthesized, characterized and their DNA crosslinking ability, photocytotoxicity in visible light and cellular localization in cancer cells studied. The bromide derivative of 2, viz. [VO(An-tpy-TPP)Br2]Br (3) is structurally characterized. The structure showed trans disposition of two halides in the coordination sphere and the TPP+ unit is a pendant to the terpyridyl ligand. The DNA melting and comet assay studies on the complexes suggest the formation of DNA crosslinks. Complexes 1 and 2 displayed ~ 10 fold increase in cytotoxicity on exposure to visible light (400–700 nm) when compared to those in dark in HeLa and MCF-7 cells. FACScan (Fluorescence Associated Cell Sorter Scan) analysis showed cellular apoptosis when treated with the complex in visible light in comparison to their dark controls. Fluorescence microscopic studies using complex 2 revealed its mitochondrial localization within the cancer cells.

AB - Oxovanadium(IV) complexes [VO(L1/L2)Cl2]n + (1,2) of (anthracenyl)terpyridine (An-tpy as L1 in 1, n = 0) and triphenylphosphonium-appended (anthracenyl)terpyridine (An-tpy-TPP+ as L2 in 2, n = 1) were synthesized, characterized and their DNA crosslinking ability, photocytotoxicity in visible light and cellular localization in cancer cells studied. The bromide derivative of 2, viz. [VO(An-tpy-TPP)Br2]Br (3) is structurally characterized. The structure showed trans disposition of two halides in the coordination sphere and the TPP+ unit is a pendant to the terpyridyl ligand. The DNA melting and comet assay studies on the complexes suggest the formation of DNA crosslinks. Complexes 1 and 2 displayed ~ 10 fold increase in cytotoxicity on exposure to visible light (400–700 nm) when compared to those in dark in HeLa and MCF-7 cells. FACScan (Fluorescence Associated Cell Sorter Scan) analysis showed cellular apoptosis when treated with the complex in visible light in comparison to their dark controls. Fluorescence microscopic studies using complex 2 revealed its mitochondrial localization within the cancer cells.

KW - Crystal structure

KW - DNA crosslinking

KW - Mitochondria targeting

KW - Photocytotoxicity

KW - Terpyridine derivative

KW - Vanadium

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DO - 10.1016/j.jinorgbio.2017.05.015

M3 - Article

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AN - SCOPUS:85020290606

SN - 0162-0134

VL - 174

SP - 45

EP - 54

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

ER -

Terpyridyl oxovanadium(IV) complexes for DNA crosslinking and mito-targeted photocytotoxicity

Abstract

Keywords

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