Terminal differentiation of human keratinocytes and stratum corneum formation is associated with caspase-14 activation

Leopold Eckhart, Wim Declercq, Jozef Ban, Michael Rendl, Barbara Lengauer, Christoph Mayer, Saskia Lippens, Peter Vandenabeele, Erwin Tschachler

Research output: Contribution to journalArticlepeer-review

187 Scopus citations

Abstract

Programmed cell death of epidermal keratinocytes (KC) results in the formation of cornified cells, which constitute the outermost skin layer, the stratum corneum. Here we show by reverse transcription-polymerase chain reaction, western blot, and immunohistochemistry that epidermal KC express caspase-14, a member of the caspase family of pro-apoptotic proteases, in a tissue-specific manner. Caspase-14 protein abundance strongly increases during terminal differentiation of KC in vivo and in vitro. Under conditions that lead to stratum corneum formation caspase-14 cleavage products, which indicate proenzyme activation, appeared in the KC lysates. Cleavage of the enzyme was also detected in lysates from normal human epidermis and in extracts of stratum corneum. Our findings demonstrate that caspase-14 is activated during KC differentiation and strongly suggest that it is involved in the formation of the human skin barrier.

Original languageEnglish
Pages (from-to)1148-1151
Number of pages4
JournalJournal of Investigative Dermatology
Volume115
Issue number6
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Apoptosis
  • Epidermal equivalent
  • Epidermis
  • Skin barrier

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