Targeting human breast tumour in xeno-grafted scid mice with 99tcm-glucarate

A. D. Petrov, J. Narula, A. Nakazawa, B. A. Khaw, B. A. Khaw

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

99Tcm-glucarate accumulation in human mammary BT-20 tumours hosted in severe combined immune deficiency (SCID) mice was compared to 111In-monoclonal antibody 103D2-F(ab’)2, 99Tcm-methoxyiso-butyl isonitrile (99Tcm-MIBI) and 99Tcm-diethylenetriamine pentaacetate (99Tcm-DTPA). The intracellular tumour distribution of 99Tcm-glucarate was also determined. SCID mice injected with a mixture of 99Tcm-glucarate and 111In-103D2-F(ab’)2 were imaged serially up until 24 h. Computer planimetered tumour-to-blood activity (in the heart) ratios (T/B11) to 8 h were significantly greater for 99Tcm-glucarate than 111In-103D2. The mean (± S.D.) tumour-to-blood ratio (T/B) from biodistribution was 1.21 ± 0.31 and 0.35 ± 0.06 (P < 0.0001) at 5 h, and 1.526 ± 0.29 and 0.75 ± 0.2 (P < 0.0001) at 8 h, for 99Tcm-glucarate and 111In-103D2 respectively. At 24 h, T/B for 111In-103D2 (1.76 ± 0.22) exceeded that of 99Tcm-glucarate (1.44 ± 0.2, P = 0.01). 99Tcm-glucarate uptake in the tumours at 5h (1.133 ± 0.25 %1D g-1) and 8h (1.213 ± 0.23 %ID g-1) was significantly greater than that of 99Tcm-MIBI (0.340 ± 0.09, P = 0.0002; 0.220 ± 0.04, P = 0.0001) and 99Tcm-DTPA (0.091 ± 0.03, P < 0.0002; 0.016 ± 0.01, P < 0.0001) respectively. Intracellular tumour distribution of 99Tcm-glucarate was 50.91 ± 6.55% in the nuclear fraction, 34.34 ± 2.88% in the cytoplasmic fraction and 14.75 ± 7.66% in the mitochondrial fraction. Thus glucarate may provide a 99Tcm-based mammary tumour imaging modality for visualization of tumours very quickly after tracer administration with maximal targeting in the nuclei of the tumour cells.

Original languageEnglish
Pages (from-to)241-251
Number of pages11
JournalNuclear Medicine Communications
Volume18
Issue number3
DOIs
StatePublished - Mar 1997
Externally publishedYes

Fingerprint

Dive into the research topics of 'Targeting human breast tumour in xeno-grafted scid mice with 99tcm-glucarate'. Together they form a unique fingerprint.

Cite this