Targeting embryonic stem cells

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations

Abstract

Gene targeting of mouse embryonic stem cells facilitates the deliberate engineering of the mouse genome. The gene targeting vector is introduced into cells by electroporation, and cell clones carrying the targeting vector are obtained by drug selection. Clones can be screened effectively for correct recombination events by either Southern blot analysis with external and internal probes, or by long-range PCR. Positive clones are subsequently expanded and prepared for the generation of chimeric mice.

Original languageEnglish
Title of host publicationTransgenesis Techniques
Subtitle of host publicationPrinciples and Protocols
EditorsElizabeth Cartwright
Pages185-197
Number of pages13
DOIs
StatePublished - 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume561
ISSN (Print)1064-3745

Keywords

  • ES cells
  • Gene Targeting
  • Long-range PCR
  • Southern blot analysis

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