TY - JOUR
T1 - Tape strips detect molecular alterations and cutaneous biomarkers in skin of patients with hidradenitis suppurativa
AU - Navrazhina, Kristina
AU - Renert-Yuval, Yael
AU - Khattri, Saakshi
AU - Hamade, Hassan
AU - Meariman, Marguerite
AU - Andrews, Elizabeth
AU - Kim, Madeline
AU - NandyMazumdar, Monali
AU - Gour, Digpal S.
AU - Bose, Swaroop
AU - Williams, Samuel C.
AU - Garcet, Sandra
AU - Correa da Rosa, Joel
AU - Gottlieb, Alice B.
AU - Krueger, James G.
AU - Guttman-Yassky, Emma
N1 - Publisher Copyright:
© 2023 American Academy of Dermatology, Inc.
PY - 2024/4
Y1 - 2024/4
N2 - Background: Hidradenitis suppurativa (HS) has a high unmet need for better treatments. Biopsies are considered the gold standard for studying molecular alterations in skin. A reproducible, minimally invasive approach is needed for longitudinal monitoring in trials and in pediatric populations. Objective: To determine whether skin tape strips can detect molecular alterations in HS and identify biomarkers of disease activity. Methods: We performed RNA sequencing on tape strips collected from lesional and healthy-appearing (nonlesional) HS skin (n = 22) and healthy controls (n = 21). We correlated the expression of skin biomarkers between tape strips and a previously published gene-signature of HS biopsies. Results: Tape strips detected upregulation of known HS biomarkers (eg, Interleukin[IL]-17A) in nonlesional and/or lesional skin and also identified novel clinically actionable targets, including OX40 and JAK3. The expression of Th17 and tumor necrosis factor-α pathways were highly correlated between tape strips and biopsies. HS clinical severity was significantly associated with expression of biomarkers (eg tumor necrosis factor-α, IL-17 A/F, OX40, JAK1-3, IL-4R) in HS lesional and/or nonlesional skin. Limitations: Sample size. Tape stripping is limited in depth. Conclusion: This study validates tape strips as a minimally-invasive approach to identify cutaneous biomarkers in HS. This provides a novel avenue for monitoring treatment efficacy and a potential step toward individualized therapy in HS.
AB - Background: Hidradenitis suppurativa (HS) has a high unmet need for better treatments. Biopsies are considered the gold standard for studying molecular alterations in skin. A reproducible, minimally invasive approach is needed for longitudinal monitoring in trials and in pediatric populations. Objective: To determine whether skin tape strips can detect molecular alterations in HS and identify biomarkers of disease activity. Methods: We performed RNA sequencing on tape strips collected from lesional and healthy-appearing (nonlesional) HS skin (n = 22) and healthy controls (n = 21). We correlated the expression of skin biomarkers between tape strips and a previously published gene-signature of HS biopsies. Results: Tape strips detected upregulation of known HS biomarkers (eg, Interleukin[IL]-17A) in nonlesional and/or lesional skin and also identified novel clinically actionable targets, including OX40 and JAK3. The expression of Th17 and tumor necrosis factor-α pathways were highly correlated between tape strips and biopsies. HS clinical severity was significantly associated with expression of biomarkers (eg tumor necrosis factor-α, IL-17 A/F, OX40, JAK1-3, IL-4R) in HS lesional and/or nonlesional skin. Limitations: Sample size. Tape stripping is limited in depth. Conclusion: This study validates tape strips as a minimally-invasive approach to identify cutaneous biomarkers in HS. This provides a novel avenue for monitoring treatment efficacy and a potential step toward individualized therapy in HS.
KW - IL-17
KW - RNA-sequencing
KW - TNF
KW - biomarkers
KW - hidradenitis suppurativa
KW - inflammation
KW - tape strips
KW - transcriptome
UR - http://www.scopus.com/inward/record.url?scp=85181112803&partnerID=8YFLogxK
U2 - 10.1016/j.jaad.2023.11.048
DO - 10.1016/j.jaad.2023.11.048
M3 - Article
C2 - 38049071
AN - SCOPUS:85181112803
SN - 0190-9622
VL - 90
SP - 749
EP - 758
JO - Journal of the American Academy of Dermatology
JF - Journal of the American Academy of Dermatology
IS - 4
ER -